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Adenine derivatization for LC-MS/MS epigenetic DNA modifications studies on monocytic THP-1 cells exposed to reference particulate matter
- Source :
- Anal. Biochem. 618:114127 (2021)
- Publication Year :
- 2021
- Publisher :
- Academic Press Inc Elsevier Science, 2021.
-
Abstract
- The aim of this study was to explore the impact of three different standard reference particulate matter (ERM-CZ100, SRM-1649, and SRM-2975) on epigenetic DNA modifications including cytosine methylation, cytosine hydroxymethylation, and adenine methylation. For the determination of low levels of adenine methylation, we developed and applied a novel DNA nucleobase chemical derivatization and combined it with liquid chromatography tandem mass spectrometry. The developed method was applied for the analysis of epigenetic modifications in monocytic THP-1 cells exposed to the three different reference particulate matter for 24 h and 48 h. The mass fraction of epigenetic active elements As, Cd, and Cr was analyzed by inductively coupled plasma mass spectrometry. The exposure to fine dust ERM-CZ100 and urban dust SRM-1649 decreased cytosine methylation after 24 h exposure, whereas all 3 p.m. increased cytosine hydoxymethylation following 24 h exposure, and the epigenetic effects induced by SRM-1649 and diesel SRM-2975 were persistent up to 48 h exposure. The road tunnel dust ERM-CZ100 significantly increased adenine methylation following the shorter exposure time. Two-dimensional scatters analysis between different epigenetic DNA modifications were used to depict a significantly negative correlation between cytosine methylation and cytosine hydroxymethylation supporting their possible functional relationship. Metals and polycyclic aromatic hydrocarbons differently shapes epigenetic DNA modifications.
- Subjects :
- Epigenomics
THP-1 Cells
Biophysics
01 natural sciences
Biochemistry
Epigenesis, Genetic
Nucleobase
Lc-ms/ms
Adenine Methylation
Chemical Derivatization
Cytosine Hydroxymethylation
Cytosine Methylation
Particulate Matter
03 medical and health sciences
chemistry.chemical_compound
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
Humans
Epigenetics
Polycyclic Aromatic Hydrocarbons
Derivatization
Molecular Biology
Inductively coupled plasma mass spectrometry
030304 developmental biology
0303 health sciences
Adenine
010401 analytical chemistry
Cell Biology
DNA Methylation
0104 chemical sciences
chemistry
DNA methylation
Cytosine
DNA
Chromatography, Liquid
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Anal. Biochem. 618:114127 (2021)
- Accession number :
- edsair.doi.dedup.....4b08808389c7cca4895a11bbf7c45925