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Vasorin deficiency leads to cardiac hypertrophy by targeting MYL7 in young mice
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2021
- Publisher :
- John Wiley and Sons Inc., 2021.
-
Abstract
- Vasorin (VASN) is an important transmembrane protein associated with development and disease. However, it is not clear whether the death of mice with VASN deficiency (VASN −/−) is related to cardiac dysfunction. The aim of this research was to ascertain whether VASN induces pathological cardiac hypertrophy by targeting myosin light chain 7 (MYL7). VASN −/− mice were produced by CRISPR/Cas9 technology and inbreeding. PCR amplification, electrophoresis, real‐time PCR and Western blotting were used to confirm VASN deficiency. Cardiac hypertrophy was examined by blood tests, histological analysis and real‐time PCR, and key downstream factors were identified by RNA sequencing and real‐time PCR. Western blotting, immunohistochemistry and electron microscopy analysis were used to confirm the downregulation of MYL7 production and cardiac structural changes. Our results showed that sudden death of VASN −/− mice occurred 21–28 days after birth. The obvious increases in cardiovascular risk, heart weight and myocardial volume and the upregulation of hypertrophy marker gene expression indicated that cardiac hypertrophy may be the cause of death in young VASN −/− mice. Transcriptome analysis revealed that VASN deficiency led to MYL7 downregulation, which induced myocardial structure abnormalities and disorders. Our results revealed a pathological phenomenon in which VASN deficiency may lead to cardiac hypertrophy by downregulating MYL7 production. However, more research is necessary to elucidate the underlying mechanism.
- Subjects :
- medicine.medical_specialty
Myosin light-chain kinase
Myosin Light Chains
Blotting, Western
Cardiomegaly
Sudden death
Muscle hypertrophy
Transcriptome
Mice
Downregulation and upregulation
Internal medicine
MYL7
medicine
Animals
Myocytes, Cardiac
Vasorin
business.industry
cardiac hypertrophy
Gene Expression Profiling
Membrane Proteins
Cell Biology
Original Articles
deficiency
Up-Regulation
Blot
Endocrinology
Molecular Medicine
Immunohistochemistry
Original Article
business
Apoptosis Regulatory Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 15824934 and 15821838
- Volume :
- 26
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....4b0052b3548e60d12a2656e291ea2193