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Nicotine activation of neuronal nitric oxide synthase and guanylyl cyclase in the medulla increases blood flow of the common carotid artery in cats
- Source :
- Neuroscience Letters. 486:122-126
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Individual activation of nicotinic acetylcholine receptor (nAChR) or nitric oxide (NO) synthase in the dorsal facial area (DFA) increases blood flow of common carotid artery (CCA) supplying intra- and extra-cranial tissues. We investigated whether the activation of nAChR initiated the activation of NO synthase and guanylyl cyclase to increase CCA blood flow in anesthetized cats. Microinjections of nicotine (a non-selective nAChR agonist), or choline (a selective α7-nAChR agonist) in the DFA produced increases in CCA blood flow ipsilaterally. These increases were significantly reduced by pretreatment with NG-nitro-arginine methyl ester (l-NAME, a non-specific NO synthase inhibitor), 7-nitroindazole (7-NI, a relatively selective neuronal NO synthase inhibitor) or methylene blue (MB, a guanylyl cyclase inhibitor) but not by that with N5-(1-iminoethyl)-l-ornithine (l-NIO, a potent endothelial NO synthase inhibitor). Control microinjection with d-NAME (an isomer of l-NAME), artificial cerebrospinal fluid or DMSO (a solvent for 7-NI) did not affect resting CCA blood flow, nor did they affect nicotine- or choline-induced response. In conclusion, activation of nAChR, at least α7-nAChR, led to the activation of neuronal NO synthase and guanylyl cyclase in the DFA, which induced an increase in CCA blood flow.
- Subjects :
- Male
Agonist
Nicotine
medicine.medical_specialty
Microinjections
alpha7 Nicotinic Acetylcholine Receptor
Carotid Artery, Common
medicine.drug_class
Hemodynamics
Nitric Oxide Synthase Type I
Receptors, Nicotinic
Nitric oxide
chemistry.chemical_compound
Internal medicine
medicine
Animals
Nicotinic Agonists
Microinjection
Acetylcholine receptor
Neurons
Medulla Oblongata
biology
Chemistry
General Neuroscience
Enzyme Activation
Nitric oxide synthase
Nicotinic acetylcholine receptor
Endocrinology
Guanylate Cyclase
Cerebrovascular Circulation
Cats
biology.protein
Female
medicine.drug
Subjects
Details
- ISSN :
- 03043940
- Volume :
- 486
- Database :
- OpenAIRE
- Journal :
- Neuroscience Letters
- Accession number :
- edsair.doi.dedup.....4afb7ad51e7bec8a8eb3fbed9641d1c0
- Full Text :
- https://doi.org/10.1016/j.neulet.2010.08.072