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The current Indian epidemic of superficial dermatophytosis due to Trichophyton mentagrophytes-A molecular study
- Source :
- Mycoses. 62(4)
- Publication Year :
- 2018
-
Abstract
- The disease burden of chronic-relapsing and therapy-refractory superficial dermatophytosis dramatically increased in India within the past 5-6 years. In order to evaluate the prevalence of this trend, 201 skin scrapings were collected from patients from all parts of India and were tested for dermatophytes using both fungal culture and a PCR-ELISA directly performed with native skin scrapings. Fungal culture material was identified by genomic Sanger sequencing of the internal transcribed spacer (ITS) region and the translation elongation factor (TEF)-1α gene. In total, 149 (74.13%) out of the 201 samples showed a dermatophyte-positive culture result. Out of this, 138 (92.62%) samples were identified as Trichophyton (T.) mentagrophytes and 11 (7.38%) as Trichophyton rubrum. The PCR-ELISA revealed similar results: 162 out of 201 (80.56%) samples were dermatophyte-positive showing 151 (93.21%) T mentagrophytes- and 11 (6.79%) T rubrum-positive samples. In this study, we show for the first time a dramatic Indian-wide switch from T rubrum to T mentagrophytes. Additionally, sequencing revealed a solely occurring T mentagrophytes "Indian ITS genotype" that might be disseminated Indian-wide due to the widespread abuse of topical clobetasol and other steroid molecules mixed with antifungal and antibacterial agents.
- Subjects :
- 0301 basic medicine
Adult
Male
Adolescent
030106 microbiology
India
Enzyme-Linked Immunosorbent Assay
Dermatology
Trichophyton rubrum
Polymerase Chain Reaction
law.invention
Microbiology
030207 dermatology & venereal diseases
03 medical and health sciences
symbols.namesake
Young Adult
0302 clinical medicine
Peptide Elongation Factor 1
Tinea
Trichophyton
law
Genotype
DNA, Ribosomal Spacer
Prevalence
Humans
Internal transcribed spacer
Child
DNA, Fungal
Epidemics
Polymerase chain reaction
Aged
Sanger sequencing
Molecular Epidemiology
biology
Molecular epidemiology
Fungal genetics
General Medicine
Sequence Analysis, DNA
Middle Aged
biology.organism_classification
Infectious Diseases
Child, Preschool
symbols
Female
Subjects
Details
- ISSN :
- 14390507
- Volume :
- 62
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Mycoses
- Accession number :
- edsair.doi.dedup.....4af378c6901ea49ae4b2030d3a4ce7dd