Back to Search
Start Over
In vivo-mimicking microfluidic perfusion culture of pancreatic islet spheroids
- Source :
- Science Advances
- Publication Year :
- 2019
-
Abstract
- Microenvironmental control of interstitial flow around pancreatic islets effectively maintains islet function in vitro.<br />Native pancreatic islets interact with neighboring cells by establishing three-dimensional (3D) structures, and are surrounded by perfusion at an interstitial flow level. However, flow effects are generally ignored in islet culture models, although cell perfusion is known to improve the cell microenvironment and to mimic in vivo physiology better than static culture systems. Here, we have developed functional islet spheroids using a microfluidic chip that mimics interstitial flow conditions with reduced shear cell damage. Dynamic culture, compared to static culture, enhanced islet health and maintenance of islet endothelial cells, reconstituting the main component of islet extracellular matrix within spheroids. Optimized flow condition allowed localization of secreted soluble factors near spheroids, facilitating diffusion-mediated paracrine interactions within islets, and enabled long-term maintenance of islet morphology and function for a month. The proposed model can aid islet preconditioning before transplantation and has potential applications as an in vitro model for diabetic drug testing.
- Subjects :
- Male
endocrine system
endocrine system diseases
Cell Culture Techniques
Islets of Langerhans Transplantation
02 engineering and technology
Models, Biological
Rats, Sprague-Dawley
03 medical and health sciences
Paracrine signalling
Islets of Langerhans
Perfusion Culture
Engineering
In vivo
Lab-On-A-Chip Devices
Spheroids, Cellular
medicine
Animals
Hypoglycemic Agents
Research Articles
030304 developmental biology
0303 health sciences
geography
Multidisciplinary
geography.geographical_feature_category
Chemistry
Pancreatic islets
Spheroid
SciAdv r-articles
Cell Biology
021001 nanoscience & nanotechnology
Islet
Cell biology
Rats
Transplantation
medicine.anatomical_structure
Cell culture
Drug Evaluation
0210 nano-technology
Research Article
Subjects
Details
- ISSN :
- 23752548
- Volume :
- 5
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Science advances
- Accession number :
- edsair.doi.dedup.....4aed9b7f7bafc2afd67ab353f3e80808