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An isotope dilution LC–MS/MS-based candidate reference method for the quantification of androstenedione in human serum and plasma
- Source :
- Clinical Mass Spectrometry
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Highlights • LC-MS/MS-based candidate reference method for the quantification of androstenedione. • Certified reference material from NMIA with additional qNMR characterization. • Uncertainty evaluation according to the GUM 1995. • Inter laboratory comparison study and comparison to a routine LC-MS/MS assay.<br />The accurate measurement of androstenedione in human serum and plasma is required for steroid profiling to assure the appropriate diagnosis and differential diagnosis of hyperandrogenism. In this work, we introduce an isotope dilution liquid chromatography–tandem mass spectrometry (LC–MS/MS) candidate reference measurement procedure for the quantification of androstenedione in human serum and plasma. The performance of the procedure enables its use in the evaluation and standardization of routine assays and for the evaluation of patient samples to ensure the traceability of individual patient results. As the primary standard, a certified reference material from NMIA (National Measurement Institute, Australia) was used. Additionally, a quantitative nuclear magnetic resonance (qNMR) method was developed for the value assignment of the primary reference material, which ensures the direct traceability to SI units, as well as the independence from the availability of reference materials. 13C3-labeled androstenedione was used as the internal standard. The introduced method allows the measurement of androstenedione in the range of 0.05–12 ng/mL, and the assay imprecision was found to be
- Subjects :
- 0303 health sciences
Chromatography
Chemistry
030302 biochemistry & molecular biology
010401 analytical chemistry
Androstenedione
Human serum
Isotope dilution
Mass spectrometry
01 natural sciences
Article
0104 chemical sciences
03 medical and health sciences
Reference measurement procedure
Certified reference materials
Reference measurement
LC–MS/MS
Primary standard
Lc ms ms
Value assignment
Quantitative NMR
Spectroscopy
Subjects
Details
- Language :
- English
- ISSN :
- 23769998 and 22138005
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Clinical Mass Spectrometry
- Accession number :
- edsair.doi.dedup.....4ae885ed714196a46bc47d015e793cf0