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Downregulation of mRNA expression of Edg-3, a putative sphingosine 1-phosphate receptor coupled to Ca2+ signaling, during differentiation of HL-60 leukemia cells

Authors :
Hideaki Tomura
Michio Ui
Fumikazu Okajima
Junko Kon
Mizuho Osada
Hiromi Nochi
Yukiko Tokumitsu
Koichi Sato
Koichi Tamoto
Hideo Ohta
Naoya Murata
Source :
Biochemical and biophysical research communications. 253(2)
Publication Year :
1999

Abstract

We measured the mRNA expression of the recently identified putative sphingosine 1-phosphate (S1P) receptors, i.e., Edg-1, AGR16/H218, and Edg-3, in HL-60 leukemia cells. Of these putative receptors, Edg-3 mRNA was abundantly expressed in undifferentiated HL-60 cells. Further, its mRNA expression was markedly downregulated by inducers of cell differentiation such as dibutyryl cAMP, retinoic acid, and 1alpha, 25-dihydroxyvitamin D3. The reduction of mRNA expression was associated with the attenuation of an S1P-induced increase in cytoplasmic free Ca2+ concentration. Thus, Edg-3, whose mRNA expression is downregulated during cell differentiation, may be responsible for the S1P-induced Ca2+ response in HL-60 leukemia cells.

Details

ISSN :
0006291X
Volume :
253
Issue :
2
Database :
OpenAIRE
Journal :
Biochemical and biophysical research communications
Accession number :
edsair.doi.dedup.....4ae3d9a990c435225b0cd5b30f5ad4a4