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Downregulation of mRNA expression of Edg-3, a putative sphingosine 1-phosphate receptor coupled to Ca2+ signaling, during differentiation of HL-60 leukemia cells
- Source :
- Biochemical and biophysical research communications. 253(2)
- Publication Year :
- 1999
-
Abstract
- We measured the mRNA expression of the recently identified putative sphingosine 1-phosphate (S1P) receptors, i.e., Edg-1, AGR16/H218, and Edg-3, in HL-60 leukemia cells. Of these putative receptors, Edg-3 mRNA was abundantly expressed in undifferentiated HL-60 cells. Further, its mRNA expression was markedly downregulated by inducers of cell differentiation such as dibutyryl cAMP, retinoic acid, and 1alpha, 25-dihydroxyvitamin D3. The reduction of mRNA expression was associated with the attenuation of an S1P-induced increase in cytoplasmic free Ca2+ concentration. Thus, Edg-3, whose mRNA expression is downregulated during cell differentiation, may be responsible for the S1P-induced Ca2+ response in HL-60 leukemia cells.
- Subjects :
- Sphingosine-1-phosphate receptor
Cellular differentiation
Biophysics
Retinoic acid
Down-Regulation
HL-60 Cells
Receptors, Cell Surface
Biology
Biochemistry
Receptors, G-Protein-Coupled
chemistry.chemical_compound
Mice
Downregulation and upregulation
NF-KappaB Inhibitor alpha
Sphingosine
medicine
Animals
Humans
Calcium Signaling
RNA, Messenger
Receptor
Molecular Biology
Messenger RNA
Cell Differentiation
Cell Biology
3T3 Cells
medicine.disease
Blotting, Northern
Molecular biology
Cell biology
DNA-Binding Proteins
Leukemia
chemistry
Receptors, Lysophospholipid
Calcium
I-kappa B Proteins
Lysophospholipids
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 253
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....4ae3d9a990c435225b0cd5b30f5ad4a4