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Terminal Deoxynucleotidyl Transferase Mediated Production of Labeled Probes for Single-molecule FISH or RNA Capture
- Source :
- Bio Protoc
- Publication Year :
- 2017
-
Abstract
- Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using basic laboratory equipment to convert arrays of PCR oligos into smFISH and RAP probesets in a quantitative, cost-efficient and flexible way.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
Chemistry
Oligonucleotide
Strategy and Management
Mechanical Engineering
Metals and Alloys
RNA
In situ hybridization
Industrial and Manufacturing Engineering
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Enzyme
Terminal deoxynucleotidyl transferase
Biochemistry
Methods Article
Molecule
Fish
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 23318325
- Volume :
- 8
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Bio-protocol
- Accession number :
- edsair.doi.dedup.....4ad1ac4dd118e233c468bb4839e75b64