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Construction of an artificial system for ambrein biosynthesis and investigation of some biological activities of ambrein
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Ambergris, a sperm whale metabolite, has long been used as a fragrance and traditional medication, but it is now rarely available. The odor components of ambergris result from the photooxidative degradation of the major component, ambrein. The pharmacological activities of ambergris have also been attributed to ambrein. However, efficient production of ambrein and odor compounds has not been achieved. Here, we constructed a system for the synthesis of ambrein and odor components. First, we created a new triterpene synthase, “ambrein synthase,” for mass production of ambrein by redesigning a bacterial enzyme. The ambrein yields were approximately 20 times greater than those reported previously. Next, an efficient photooxidative conversion system from ambrein to a range of volatiles of ambergris was established. The yield of volatiles was 8–15%. Finally, two biological activities, promotion of osteoclast differentiation and prevention of amyloid β-induced apoptosis, were discovered using the synthesized ambrein.
- Subjects :
- 0301 basic medicine
Metabolite
Osteoclasts
lcsh:Medicine
Ambergris
Apoptosis
Naphthols
010402 general chemistry
01 natural sciences
Biochemistry
Article
Cell Line
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Animals
Humans
lcsh:Science
Ambrein
Volatile Organic Compounds
Multidisciplinary
Amyloid beta-Peptides
Sperm Whale
lcsh:R
Cell Differentiation
Chemical biology
Triterpenes
0104 chemical sciences
030104 developmental biology
chemistry
Odor
Photooxidative degradation
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....4acf1e5401dbc247dc0c467012af934f