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Photoelectrochemical Imaging System for the Mapping of Cell Surface Charges
- Source :
- Analytical Chemistry
- Publication Year :
- 2019
-
Abstract
- The surface charge of cells affects cell signaling, cell metabolic processes, adherence to surfaces, and cell proliferation. Our understanding of the role of membrane charges is limited due to the inability to observe changes without interfering, chemically or physically, with the cell or its membrane. Here, we report that a photoelectrochemical imaging system (PEIS) based on label-free ac-photocurrent measurements at indium tin oxide (ITO) coated glass substrates can be used to map the basal surface charge of single live cells under physiological conditions. Cells were cultured on the ITO substrate. Photocurrent images were generated by scanning a focused, modulated laser beam across the back of the ITO coated glass substrate under an applied bias voltage. The photocurrent was shown to be sensitive to the negative surface charge of the substrate facing, basal side of a single living cell-an area not accessible to other electrochemical or electrophysiological imaging techniques. The PEIS was used to monitor the lysis of mesenchymal stem cells.
- Subjects :
- Lysis
Light
Surface Properties
Metal Nanoparticles
Substrate (electronics)
Biosensing Techniques
010402 general chemistry
01 natural sciences
Analytical Chemistry
Neuroblastoma
Electrochemistry
Animals
Humans
QD
Surface charge
Electrodes
Cells, Cultured
Photocurrent
Chemistry
010401 analytical chemistry
Tin Compounds
Biasing
Mesenchymal Stem Cells
0104 chemical sciences
Indium tin oxide
Rats
Membrane
Electrode
Biophysics
Glass
Gold
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 91
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....4abb02e9982eb5cd62e042d4d0ba9511