Back to Search
Start Over
The Ankyrin Repeat Domain 49 (ANKRD49) Augments Autophagy of Serum-Starved GC-1 Cells through the NF-κB Pathway
- Source :
- PLoS ONE, Vol 10, Iss 6, p e0128551 (2015), PLoS ONE
- Publication Year :
- 2015
- Publisher :
- Public Library of Science (PLoS), 2015.
-
Abstract
- The ankyrin repeat domain 49 (ANKRD49) is an evolutionarily conserved protein highly expressed in testes. However, the function of ANKRD49 in spermatogenesis is unknown. In this study, we found that ANKRD49 resides primarily in nucleus of spermatogonia, spermatocytes and round spermatids. ANKRD49 overexpression augments starvation-induced autophagy in male germ GC-1 cells whereas shRNA knockdown of ANKRD49 attenuates the autophagy. Inhibition of NF-κB pathway by its inhibitors or p65 siRNA prevents the ANKRD49-dependent autophagy augmentation, demonstrating that ANKRD49 enhances autophagy via NF-κB pathway. Our findings suggest that ANKRD49 plays an important role in spermatogenesis via promotion of autophagy-dependent survival.
- Subjects :
- Male
Time Factors
Transcription, Genetic
Molecular Sequence Data
Muscle Proteins
lcsh:Medicine
Biology
BAG3
Prophase
Culture Media, Serum-Free
Cell Line
chemistry.chemical_compound
Testis
Autophagy
Animals
Luciferase
Amino Acid Sequence
Spermatogenesis
lcsh:Science
Transcription factor
Mice, Inbred BALB C
Multidisciplinary
lcsh:R
NF-kappa B
Gene Expression Regulation, Developmental
NF-κB
Spermatozoa
Ankyrin Repeat
Cell biology
chemistry
Apoptosis
Gene Knockdown Techniques
Ankyrin repeat
lcsh:Q
Signal Transduction
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....4ab898bb7f0401b0e7e637d81903bc9f