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Molecular cloning, characterisation and tissues expression analysis of the goose (Anser cygnoides) vasoactive intestinal peptide (VIP) gene
- Source :
- British Poultry Science. 55:720-727
- Publication Year :
- 2014
- Publisher :
- Informa UK Limited, 2014.
-
Abstract
- 1. Vasoactive intestinal peptide (VIP) is involved in the control of prolactin (PRL) release and plays a pivotal role as a regulator of reproductive behaviour and neuroendocrine secretion in birds. 2. In this study, a 941-bp cDNA fragment covering the complete coding region (CDS) of goose VIP gene was identified. The cDNA contains a 32-bp 5'-untranslated region (UTR), a 603-bp CDS and a 306-bp 3'-UTR containing two ATTTA sequence elements, two polyadenylation signals (AATAAA) and a 25-bp poly (A) tail. 3. Seven exons and 6 introns were identified, and both the cDNA and genomic DNA sequences showed high identity with those of other species. 4. The sequence analysis indicated that there were two alternatively spliced transcripts the long transcript (VIP-1) encoded both VIP and peptide histidine isoleucine exons and the short one (VIP-2) only encoded VIP. 5. RT-PCR analysis indicates that the expression level of the VIP-1 is much lower than that of VIP-2, and that VIP-1 is negligible or absent in muscle, abdominal fat, ovary and spleen, whereas VIP-2 is widely distributed in all the examined tissues. 6. A total of 12 single nucleotide polymorphisms (SNPs), including 2 SNPs located in the coding region and 10 variations in intron regions, were identified in goose VIP gene.
- Subjects :
- Untranslated region
Sequence analysis
Molecular Sequence Data
Vasoactive intestinal peptide
Biology
Polymorphism, Single Nucleotide
Exon
Complementary DNA
Geese
Animals
Coding region
Tissue Distribution
Amino Acid Sequence
Cloning, Molecular
Peptide sequence
Phylogeny
Genetics
Base Sequence
Intron
General Medicine
Molecular biology
Female
Animal Science and Zoology
hormones, hormone substitutes, and hormone antagonists
Vasoactive Intestinal Peptide
Food Science
Subjects
Details
- ISSN :
- 14661799 and 00071668
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- British Poultry Science
- Accession number :
- edsair.doi.dedup.....4ab751de8f914659f8343513cdf328d8