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Genetic and antigenic diversity among noroviruses
- Source :
- Journal of General Virology. 87:909-919
- Publication Year :
- 2006
- Publisher :
- Microbiology Society, 2006.
-
Abstract
- Human norovirus (NoV) strains cause a considerable number of outbreaks of gastroenteritis worldwide. Based on their capsid gene (VP1) sequence, human NoV strains can be grouped into two genogroups (GI and GII) and at least 14 GI and 17 GII genotypes (GI/1–14 and GII/1–17). Human NoV strains cannot be propagated in cell-culture systems, but expression of recombinant VP1 in insect cells results in the formation of virus-like particles (VLPs). In order to understand NoV antigenic relationships better, cross-reactivity among 26 different NoV VLPs was analysed. Phylogenetic analyses grouped these NoV strains into six GI and 12 GII genotypes. An antibody ELISA using polyclonal antisera raised against these VLPs was used to determine cross-reactivity. Antisera reacted strongly with homologous VLPs; however, a number of novel cross-reactivities among different genotypes was observed. For example, GI/11 antiserum showed a broad-range cross-reactivity, detecting two GI and 10 GII genotypes. Likewise, GII/1, GII/10 and GII/12 antisera showed a broad-range cross-reactivity, detecting several other distinct GII genotypes. Alignment of VP1 amino acid sequences suggested that these broad-range cross-reactivities were due to conserved amino acid residues located within the shell and/or P1-1 domains. However, unusual cross-reactivities among different GII/3 antisera were found, with the results indicating that both conserved amino acid residues and VP1 secondary structures influence antigenicity.
- Subjects :
- Antigenicity
Genotype
viruses
Molecular Sequence Data
Cross Reactions
Antibodies, Viral
medicine.disease_cause
Microbiology
fluids and secretions
Virology
medicine
Humans
Amino Acid Sequence
Phylogeny
Antiserum
Phylogenetic tree
biology
Norovirus
Virion
Genetic Variation
virus diseases
Antigenic Variation
Capsid
Polyclonal antibodies
biology.protein
Capsid Proteins
Antibody
Sequence Alignment
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....4aaf6f078250db8ba3810ee49fc2d504