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Increased phospholipase D2 activity during hypoxia-induced death of PC12 cells: its possible anti-apoptotic role
- Source :
- Neuroreport. 11(16)
- Publication Year :
- 2000
-
Abstract
- During hypoxic incubation (1% O2) of PC12 cells, the PLD activity was transiently increased within 12h, followed by a gradual decrease. In the in vitro assay, the increased PLD activity was independent of GTPgammaS required for PLD1 or of oleic acid for PLD(OA), suggesting the activation of PLD2. The level of PLD2 protein showed no change up to 12h but a gradual decrease after 24 h. Pretreatment of cells with S. chromofuscus PLD resulted in inhibition of hypoxia-induced apoptotic cell death. In contrast, 1-butanol, but not 2-butanol, potentiated cell death. Moreover, the number of apoptotic cells significantly reduced in PC12 cells over-expressing PLD2. These results raise the possibility that PLD2 activation may play an anti-apoptotic role in hypoxia-induced cell death.
- Subjects :
- Programmed cell death
Time Factors
Butanols
GTPgammaS
Apoptosis
Phospholipase
Biology
PC12 Cells
chemistry.chemical_compound
1-Butanol
Phospholipase D
Animals
Cell Death
General Neuroscience
PLD2
Cell Hypoxia
Streptomyces
Cell biology
Rats
Kinetics
chemistry
Biochemistry
Cell culture
Guanosine 5'-O-(3-Thiotriphosphate)
lipids (amino acids, peptides, and proteins)
Signal transduction
Subjects
Details
- ISSN :
- 09594965
- Volume :
- 11
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- Neuroreport
- Accession number :
- edsair.doi.dedup.....4aa36debfc407ca4d5310dbb9d7a0dc2