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Genes associated with an effective host response by Chinook salmon to Renibacterium salmoninarum
- Source :
- Developmental & Comparative Immunology. 33:176-186
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- An effective host response to Renibacterium salmoninarum, the etiologic agent of bacterial kidney disease, is poorly characterized. Using suppression subtractive hybridization, we exploited the difference in early host response in the pronephros of fish challenged by an attenuated strain (MT239) or a virulent strain (ATCC 33209) of R. salmoninarum. Among the 132 expressed sequence tag (EST) clones that were sequenced, 20 were selected for expression analysis at 24 and 72h after challenge. ESTs matching two interferon inducible genes (IFN-inducible GBP and VLIG1), the ligand GAS6, and the kinase VRK2 were upregulated in fish exposed to MT239, but downregulated or unchanged in fish exposed to 33209. A second group of ESTs matching genes involved in apoptosis (caspase 8) and immune function (IkappaBalpha, p47(phoX), EMR/CD97) were more slowly upregulated in fish exposed to 33209 compared to fish exposed to MT239. The ESTs displaying elevated expression in MT239-exposed fish may represent important cellular processes to bacterial challenge, and may be useful indicators of an effective host response to R. salmoninarum infection.
- Subjects :
- Ribosomal Proteins
Genetics
Expressed sequence tag
DNA, Complementary
Transcription, Genetic
Immunology
Virulence
Biology
Phosphoproteins
medicine.disease_cause
Microbiology
Pronephros
Immune system
Salmon
Interferon
Suppression subtractive hybridization
Bacterial kidney disease
medicine
Animals
Kidney Diseases
Renibacterium salmoninarum
Actinomycetales Infections
Micrococcaceae
Developmental Biology
medicine.drug
Subjects
Details
- ISSN :
- 0145305X
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Developmental & Comparative Immunology
- Accession number :
- edsair.doi.dedup.....4aa2fc03b86e66e9266b3c048e31b402