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A field indoor air measurement of SARS-CoV-2 in the patient rooms of the largest hospital in Iran
- Source :
- Science of The Total Environment, The Science of the Total Environment
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The coronavirus disease 2019 (COVID-19) emerged in Wuhan city, China, in late 2019 and has rapidly spread throughout the world. The major route of transmission of SARS-CoV-2 is in contention, with the airborne route a likely transmission pathway for carrying the virus within indoor environments. Until now, there has been no evidence for detection of airborne severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and this may have implication for the potential spread of the COVID-19. We investigated the air of patient rooms with confirmed COVID-19 in the largest hospital in Iran, on March 17, 2020. To collect the SARS-CoV-2 particles, ten air samples were collected into the sterile standard midget impingers containing 20 mL DMEM with 100 μg/mL streptomycin, 100 U/mL penicillin and 1% antifoam reagent for 1 h. Besides, indoor particle number concentrations, CO2, relative humidity and temperature were recorded throughout the sampling duration. Viral RNA was extracted from samples taken from the impingers and Reverse-Transcription PCR (RT-PCR) was applied to confirm the positivity of collected samples based on the virus genome sequence. Fortunately, in this study all air samples which were collected 2 to 5 m from the patients' beds with confirmed COVID-19 were negative. Despite we indicated that all air samples were negative, however, we suggest further in vivo experiments should be conducted using actual patient cough, sneeze and breath aerosols in order to show the possibility of generation of the airborne size carrier aerosols and the viability fraction of the embedded virus in those carrier aerosols.<br />Graphical abstract Unlabelled Image<br />Highlights • To address the airborne transmission mode of SARS-CoV-2 air samples were collected in the largest hospital in Iran. • Our results indicated that all collected samples were negative in terms of the viral RNA. • We did not detect any positive readings 2 m from the patients' beds.
- Subjects :
- Veterinary medicine
2019-20 coronavirus outbreak
China
Environmental Engineering
010504 meteorology & atmospheric sciences
Coronavirus disease 2019 (COVID-19)
Indoor air
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Pneumonia, Viral
010501 environmental sciences
Iran
01 natural sciences
Article
Betacoronavirus
Patients' Rooms
Humans
Environmental Chemistry
Viral rna
Pandemics
Waste Management and Disposal
0105 earth and related environmental sciences
SARS-CoV-2
Outbreak
COVID-19
Pollution
Patient room
Air Pollution, Indoor
Airborne
Environmental science
Coronavirus Infections
Tehran
Subjects
Details
- Language :
- English
- ISSN :
- 00489697
- Database :
- OpenAIRE
- Journal :
- Science of The Total Environment
- Accession number :
- edsair.doi.dedup.....4a9e42e03a1156cc6ea3b800f669ee51
- Full Text :
- https://doi.org/10.1016/j.scitotenv.2020.138401