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In Vitro Sensitivity of Cutaneous Leishmania Promastigote Isolates Circulating in French Guiana to a Set of Drugs
- Source :
- American Journal of Tropical Medicine and Hygiene, American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2016, 29 (4), pp.16-0373. ⟨10.4269/ajtmh.16-0373⟩, American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2017, [Epub ahead of print]. ⟨10.4269/ajtmh.16-0373⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- International audience; Anti-leishmaniasis drug resistance is a common problem worldwide. The aim of this study was to inventory the general in vitro level of sensitivity of Leishmania isolates circulating in French Guiana and to highlight potential in vitro pentamidine-resistant isolates. This sensitivity study was conducted on 36 patient-promastigote isolates for seven drugs (amphotericin B, azithromycin, fluconazole, meglumine antimoniate, miltefosine, paromomycin, and pentamidine) using the Cell Counting Kit-8 viability test. The IC50 values obtained were heterogeneous. One isolate exhibited high IC50 values for almost all drugs tested. Pentamidine, which is the first-line treatment in French Guiana, showed efficacy at very low doses (mean of 0.0038 μg/mL). The concordance of the in vitro pentamidine results with the patients' clinical outcomes was 94% (K = 0.82).
- Subjects :
- 0301 basic medicine
Meglumine antimoniate
030231 tropical medicine
030106 microbiology
Paromomycin
Drug resistance
Pharmacology
Biology
03 medical and health sciences
0302 clinical medicine
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Virology
Amphotericin B
parasitic diseases
medicine
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
ComputingMilieux_MISCELLANEOUS
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Miltefosine
[SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE]
Leishmaniasis
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
medicine.disease
biology.organism_classification
Leishmania braziliensis
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
Infectious Diseases
[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Parasitology
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
medicine.drug
Pentamidine
Subjects
Details
- Language :
- English
- ISSN :
- 00029637
- Database :
- OpenAIRE
- Journal :
- American Journal of Tropical Medicine and Hygiene, American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2016, 29 (4), pp.16-0373. ⟨10.4269/ajtmh.16-0373⟩, American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2017, [Epub ahead of print]. ⟨10.4269/ajtmh.16-0373⟩
- Accession number :
- edsair.doi.dedup.....4a9e11f1706526b4b24a7ebb8c60b23f