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[Effects of traditional Tibetan drug Liu Tea on proliferation and chemotherapeutic sensitivity of drug-resistant human gastric cancer cell BGC823/5-FU]
- Source :
- Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 41(4)
- Publication Year :
- 2015
-
Abstract
- To investigate the effects of Liu Tea extracts(LTE) on proliferation, apoptosis and drug sensitivity of drug-resistant gastric cancer cell BGC823/5-FU. MTT assay was used to analyze effect of LTE on cell growth and sensitivity chemotherapeutic drugs, and synergistic effect of the combination of LTE with 5-FU on BGC823/5-FU cells. Combination index (CI) was calculated by CompuSyn. Cell apoptosis was measured by flow cytometry (FCM). Protein expressions of P-gp, Bcl-2, Bax and Caspase-3 (17KD) were detected by Western blot at different concentrations of LTE in BGC823/5-FU cells (100, 200, 400 mg•L⁻¹). The results showed that LTE had an inhibitory effect on growth of BGC823/5-FU cell in a dose-time-dependent manner and significantly reduced IC₅₀ of 5-FU, CDDP, PTX and ADM to BGC823/5-FU cells(P
- Subjects :
- Cell
Antineoplastic Agents
Apoptosis
Tibet
Flow cytometry
Western blot
Stomach Neoplasms
Cell Line, Tumor
medicine
Humans
Pharmacology (medical)
MTT assay
General Pharmacology, Toxicology and Pharmaceutics
Cell Proliferation
medicine.diagnostic_test
Cell growth
Chemistry
Caspase 3
Cell Cycle
medicine.anatomical_structure
Complementary and alternative medicine
Cell culture
Cancer research
Fluorouracil
Apoptosis Regulatory Proteins
Reverse tolerance
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 10015302
- Volume :
- 41
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
- Accession number :
- edsair.doi.dedup.....4a9b7877034b1046fb2eb2b810008154