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Lipopolysaccharide as trigger of platelet aggregation via eicosanoid over-production
- Source :
- Thrombosis and haemostasis. 117(8)
- Publication Year :
- 2016
-
Abstract
- SummaryThe effect of lipopolysaccharide (LPS) on platelet aggregation is still controversial. We performed in vitro and ex vivo studies in controls and in patients with community-acquired pneumonia (CAP) to assess the effect of LPS on platelet activation (PA). LPS (15–100 pg/ml) significantly increased PA only if combined with sub-threshold concentrations (STC) of collagen or ADP; this effect was associated with increased platelet H2O2 production, Nox2 activation, PLA2 phosphorylation, thromboxane (Tx)A2 and 8-iso-PGF2α-III, and was inhibited by aspirin, TxA2 receptor antagonist or by Toll-like receptor 4 blocking peptide (TLR4bp). Analysis of up-stream signalling potentially responsible for Nox2 and PLA2 activation demonstrated that LPS-mediated PA was associated with phosphorylation of AKT, p38 and p47phox translocation. In 10 consecutive CAP patients serum endotoxins were significantly higher compared to 10 controls (145 [115–187] vs 18 [6–21] pg/ml; pSupplementary Material to this article is available online at www.thrombosis-online.com
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Male
Time Factors
Lipopolysaccharide
Platelet Aggregation
Thromboxane
030204 cardiovascular system & hematology
Dinoprost
p38 Mitogen-Activated Protein Kinases
chemistry.chemical_compound
0302 clinical medicine
Platelet
Phosphorylation
Receptor
Hematology
Middle Aged
Receptor antagonist
Community-Acquired Infections
NADPH Oxidase 2
lipids (amino acids, peptides, and proteins)
Female
LPS
community-acquired pneumonia
oxidative stress
platelet activation
Adult
Blood Platelets
medicine.medical_specialty
medicine.drug_class
03 medical and health sciences
Thromboxane A2
Internal medicine
medicine
Pneumonia, Bacterial
Humans
Platelet activation
Calcium Signaling
Aged
Dose-Response Relationship, Drug
Group IV Phospholipases A2
NADPH Oxidases
Hydrogen Peroxide
Enzyme Activation
Toll-Like Receptor 4
Oxidative Stress
030104 developmental biology
Endocrinology
chemistry
Eicosanoid
Case-Control Studies
Proto-Oncogene Proteins c-akt
Ex vivo
Subjects
Details
- ISSN :
- 2567689X
- Volume :
- 117
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Thrombosis and haemostasis
- Accession number :
- edsair.doi.dedup.....4a97899bd63ffcca153f2a577d033a14