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Anti-herpes simplex virus activities of monogalactosyl diglyceride and digalactosyl diglyceride from Clinacanthus nutans, a traditional Thai herbal medicine
- Source :
- Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 3, Pp 192-197 (2016)
- Publication Year :
- 2016
- Publisher :
- Medknow, 2016.
-
Abstract
- Objective To evaluate the monogalactosyl diglyceride (MGDG) and digalactosyl diglyceride (DGDG) from Clinacanthus nutans ( C. nutans ) for their in vitro antiviral activities against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) by plaque reduction assay. Methods MGDG and DGDG were extracted with chloroform from C. nutans leaves. MGDG and DGDG were separated from chloroform crude extract using column chromatography, characterized by thin layer chromatography and quantified by high performance liquid chromatography. The anti HSV-1 and 2 activity against pre-treatment and post-treatment of the compounds was evaluated using plaque reduction assay. The cytotoxicity of the extract and the compounds on Vero cells were performed by MTT assay. Results MGDG and DGDG obtained by column chromatography showed identical profiles as standard MGDG and standard DGDG using thin layer chromatography and high performance liquid chromatography. MGDG and DGDG from C. nutans showed 100% inhibition of HSV-1 replication at the post step of infection at noncytotoxic concentration with IC 50 values of 36.00 and 40.00 μg/mL, and HSV-2 at 41.00 and 43.20 μg/mL, respectively. Moreover, MGDG and DGDG from C. nutans were demonstrated to have anti-herpes simplex activity at the same level as standard synthetic compounds. In contrast, pre-treatment of Vero cells with MGDG and DGDG before HSV-1 and HSV-2 infection did not show inhibitory effect against these viruses. MGDG and DGDG exhibited antiviral activity against HSV-1 with selectivity index of 26.00 and 23.00 and HSV-2 of 23.30 and 21.30. Conclusions MGDG and DGDG from C. nutans , a traditional Thai herbal medicine illustrated inhibitory activity against HSV-1 and HSV-2, probably by inhibiting the late stage of multiplication, suggesting their promising use as anti-HSV agents.
- Subjects :
- 0301 basic medicine
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
viruses
Clinacanthus nutans
030106 microbiology
ved/biology.organism_classification_rank.species
Digalactosyl diglyceride
Herpes simplex virus type 2
Herpes simplex virus type 1
medicine.disease_cause
Biochemistry, Genetics and Molecular Biology (miscellaneous)
High-performance liquid chromatography
Monogalactosyl diglyceride
03 medical and health sciences
chemistry.chemical_compound
Column chromatography
medicine
MTT assay
Diglyceride
Antiviral activity
Cytotoxicity
lcsh:QH301-705.5
Chemistry
ved/biology
Thin-layer chromatography
030104 developmental biology
Herpes simplex virus
lcsh:Biology (General)
Biochemistry
Glycoglycerolipids
Subjects
Details
- ISSN :
- 22211691
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Asian Pacific Journal of Tropical Biomedicine
- Accession number :
- edsair.doi.dedup.....4a8fc87ba9d26aa0414ddf1baf92de1c
- Full Text :
- https://doi.org/10.1016/j.apjtb.2015.12.014