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Selenium Plays an Anti-Inflammatory Role by Regulation NLRP3 Inflammasome in Staphylococcus aureus-Infected Mouse Mammary Gland
- Source :
- Biological Trace Element Research. 199:604-610
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Selenium is an essential micronutrient that plays an important role in immunity. However, the mechanism that Selenium modulates mastitis is not fully clear. In this experiment, we investigated whether selenium can inhibit the activation of the NLRP3 inflammasome in a mouse model of Staphylococcus aureus-induced mastitis. Eighty BALB/c female mice were fed with experimental Selenium deficiency basal diet for 2 weeks to achieve the purpose of selenium consumption until pregnancy. Pregnant mice were randomly divided into four groups (control group; selenium supplement group; Staphylococcus aureus infection group and Staphylococcus aureus infection after selenium supplement group). Twenty-four hours after challenging, all mice were euthanized and mammary tissue samples were aseptically collected. Through pathological staining, western blot analysis, real-time fluorescence quantitative polymerase chain reaction analysis, and enzyme-linked immunosorbent assay, the regulation effect of Selenium on NLRP3 inflammasome was detected. The result showed that compared with the control group, selenium significantly inhibited the expression of NLRP3, ASC, Caspase-1, Caspase-1 p20, and Pro-IL-1β (p
- Subjects :
- Staphylococcus aureus
Inflammasomes
Endocrinology, Diabetes and Metabolism
Interleukin-1beta
Clinical Biochemistry
Anti-Inflammatory Agents
chemistry.chemical_element
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Biochemistry
Microbiology
Inorganic Chemistry
Mice
Selenium
03 medical and health sciences
Western blot
Selenium deficiency
NLR Family, Pyrin Domain-Containing 3 Protein
medicine
Animals
0105 earth and related environmental sciences
Mice, Inbred BALB C
0303 health sciences
medicine.diagnostic_test
business.industry
030302 biochemistry & molecular biology
Biochemistry (medical)
food and beverages
Inflammasome
General Medicine
Staphylococcal Infections
medicine.disease
Mastitis
Real-time polymerase chain reaction
chemistry
Female
business
Staphylococcus
medicine.drug
Subjects
Details
- ISSN :
- 15590720 and 01634984
- Volume :
- 199
- Database :
- OpenAIRE
- Journal :
- Biological Trace Element Research
- Accession number :
- edsair.doi.dedup.....4a87fcfae9093b6fc59147137b1d282f