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First Description of a Satellite DNA in Manatees’ Centromeric Regions
- Source :
- Frontiers in Genetics, Vol 12 (2021), Frontiers in Genetics
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and TAREAN to identify satellite DNAs (satDNAs) in this species. We report the first description of TMAsat, a satDNA comprising ~0.87% of the genome, with ~684bp monomers and centromeric localization. In T. inunguis, TMAsat showed similar monomer length, chromosome localization and conserved CENP-B box-like motifs as in T. manatus. We also detected this satDNA in the Dugong dugon and in the now extinct Hydrodamalis gigas genomes. The neighbor-joining tree shows that TMAsat sequences from T. manatus, T. inunguis, D. dugon, and H. gigas lack species-specific clusters, which disagrees with the predictions of concerted evolution. We detected a divergent TMAsat-like homologous sequence in elephants and hyraxes, but not in other mammals, suggesting this sequence was already present in the common ancestor of Paenungulata, and later became a satDNA in the Sirenians. This is the first description of a centromeric satDNA in manatees and will facilitate the inclusion of Sirenia in future studies of centromeres and satDNA biology.
- Subjects :
- Concerted evolution
biology
Satellite DNA
Chromosome localization
chromosome mapping
Paenungulata
Trichechus manatus
Brief Research Report
QH426-470
biology.organism_classification
Genome
tandem repeats
Trichechus inunguis
Evolutionary biology
fluorescent in situ hybridization
Genetics
Molecular Medicine
Sirenia
Genetics (clinical)
TAREAN
Subjects
Details
- Language :
- English
- ISSN :
- 16648021
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Genetics
- Accession number :
- edsair.doi.dedup.....4a7e31b6023980f6a098f1f8fbd703d1
- Full Text :
- https://doi.org/10.3389/fgene.2021.694866/full