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First Description of a Satellite DNA in Manatees’ Centromeric Regions

Authors :
Yatiyo Yonenaga-Yassuda
Alice Alves do Espírito Santo
Mirela Pelizaro Valeri
Guilherme B. Dias
Camila do Nascimento Moreira
Marta Svartman
Iara Braga Sommer
Gustavo C. S. Kuhn
Source :
Frontiers in Genetics, Vol 12 (2021), Frontiers in Genetics
Publication Year :
2021
Publisher :
Frontiers Media S.A., 2021.

Abstract

Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and TAREAN to identify satellite DNAs (satDNAs) in this species. We report the first description of TMAsat, a satDNA comprising ~0.87% of the genome, with ~684bp monomers and centromeric localization. In T. inunguis, TMAsat showed similar monomer length, chromosome localization and conserved CENP-B box-like motifs as in T. manatus. We also detected this satDNA in the Dugong dugon and in the now extinct Hydrodamalis gigas genomes. The neighbor-joining tree shows that TMAsat sequences from T. manatus, T. inunguis, D. dugon, and H. gigas lack species-specific clusters, which disagrees with the predictions of concerted evolution. We detected a divergent TMAsat-like homologous sequence in elephants and hyraxes, but not in other mammals, suggesting this sequence was already present in the common ancestor of Paenungulata, and later became a satDNA in the Sirenians. This is the first description of a centromeric satDNA in manatees and will facilitate the inclusion of Sirenia in future studies of centromeres and satDNA biology.

Details

Language :
English
ISSN :
16648021
Volume :
12
Database :
OpenAIRE
Journal :
Frontiers in Genetics
Accession number :
edsair.doi.dedup.....4a7e31b6023980f6a098f1f8fbd703d1
Full Text :
https://doi.org/10.3389/fgene.2021.694866/full