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Factors contributing to salivary human immunodeficiency virus type-1 levels measured by a Poisson distribution-based PCR method
- Source :
- Journal of International Medical Research, Vol 46 (2018), The Journal of International Medical Research
- Publication Year :
- 2018
- Publisher :
- SAGE Publishing, 2018.
-
Abstract
- Objective To elucidate the mechanism underlying secretion of human immunodeficiency virus type 1 (HIV-1) into the oral cavity, by examining the relationships between various oral and systemic factors and the viral load in saliva. Methods Plasma and saliva samples from HIV-1 infected patients were assayed using the COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, version 1.0 and a Poisson distribution-based polymerase chain reaction (PCR) method for quantifying HIV-1 RNA and DNA. Results Forty-four pairs of samples were obtained from 18 patients. Salivary viral load was approximately 10% of the plasma viral load, but higher than the plasma load in two patients. The salivary viral DNA load was Conclusions The present results suggest that excretion through salivary glands, but not occult bleeding, may be a major pathway of HIV-1 into the oral cavity.
- Subjects :
- 0301 basic medicine
Adult
Male
Saliva
Medicine (General)
Anti-HIV Agents
Human immunodeficiency virus (HIV)
HIV Infections
Biology
Poisson distribution
Oral cavity
medicine.disease_cause
Biochemistry
Polymerase Chain Reaction
Sensitivity and Specificity
law.invention
03 medical and health sciences
symbols.namesake
0302 clinical medicine
R5-920
law
Antiretroviral Therapy, Highly Active
medicine
Humans
Secretion
030212 general & internal medicine
Polymerase chain reaction
plasma
saliva
Biochemistry (medical)
HIV
Research Reports
Cell Biology
General Medicine
Middle Aged
Viral Load
Virology
quantification
030104 developmental biology
Immunology
DNA, Viral
symbols
HIV-1
RNA, Viral
Female
Pcr method
Viral load
Subjects
Details
- Language :
- English
- ISSN :
- 14732300 and 03000605
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Journal of International Medical Research
- Accession number :
- edsair.doi.dedup.....4a74504d2b947b52d7c68016ca6957dd