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Solid-phase radioassay for the determination of binding activity of solubilized tritiated Fc gamma receptor of guinea pig peritoneal macrophages
- Source :
- Journal of immunological methods. 79(1)
- Publication Year :
- 1985
-
Abstract
- The reductive methylation procedure for proteins in solution has been adapted for tritium radiolabelling of surface components of macrophages, including the Fcγ receptor. High specific radioactivity of the receptor was obtained with no loss of IgG binding activity, tested by the solid-phase radioassay. To prepare the support for the assay, cellulose filter paper was CNBr-activated and a diamine hydrocarbon reagent was attached as the spacer chain. Next, TNP-hapten residues were covalently coupled to free amine groups. Antibody-coated TNP-paper discs were used as the solid phase for the competitive binding radioassay for the determination of IgG-binding activity of purified Fcγ receptor. The developed radioassay permits the detection of low affinity interactions and/or low quantities of Fc receptors.
- Subjects :
- Paper
Immunology
Guinea Pigs
Radioimmunoassay
Receptors, Fc
Tritium
chemistry.chemical_compound
Affinity chromatography
Diamine
Immunology and Allergy
Animals
Receptor
Peritoneal Cavity
Chromatography
Chemistry
Macrophages
Receptors, IgG
Biochemistry
Solubility
IgG binding
Reagent
Immunoglobulin G
Trinitrobenzenes
Amine gas treating
PMSF
Subjects
Details
- ISSN :
- 00221759
- Volume :
- 79
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of immunological methods
- Accession number :
- edsair.doi.dedup.....4a6a200717895d95afa69c71a88a8571