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nsPEF-induced PIP2 depletion, PLC activity and actin cytoskeletal cortex remodeling are responsible for post-exposure cellular swelling and blebbing
- Source :
- Biochemistry and Biophysics Reports, Vol 9, Iss C, Pp 36-41 (2017), Biochemistry and Biophysics Reports
- Publisher :
- The Author(s). Published by Elsevier B.V.
-
Abstract
- Cell swelling and blebbing has been commonly observed following nanosecond pulsed electric field (nsPEF) exposure. The hypothesized origin of these effects is nanoporation of the plasma membrane (PM) followed by transmembrane diffusion of extracellular fluid and disassembly of cortical actin structures. This investigation will provide evidence that shows passive movement of fluid into the cell through nanopores and increase of intracellular osmotic pressure are not solely responsible for this observed phenomena. We demonstrate that phosphatidylinositol-4,5-bisphosphate (PIP2) depletion and hydrolysis are critical steps in the chain reaction leading to cellular blebbing and swelling. PIP2 is heavily involved in osmoregulation by modulation of ion channels and also serves as an intracellular membrane anchor to cortical actin and phospholipase C (PLC). Given the rather critical role that PIP2 depletion appears to play in the response of cells to nsPEF exposure, it remains unclear how its downstream effects and, specifically, ion channel regulation may contribute to cellular swelling, blebbing, and unknown mechanisms of the lasting “permeabilization” of the PM.<br />Highlights • Nanosecond electric pulses (nsEPs) of high amplitude induce hydrolysis of PIP2. • PLC activation is leading to post-exposure cellular swelling and blebbing. • Ion channels modulation and nanoporation are responsible for cellular swelling. • Cortical actin dissociation after PIP2 depletion is critical for cellular blebbing.
- Subjects :
- 0301 basic medicine
PIP2 hydrolysis
Biophysics
Biochemistry
lcsh:Biochemistry
03 medical and health sciences
Nanopores
0302 clinical medicine
Nanosecond pulsed electric field
Extracellular fluid
Osmotic pressure
lcsh:QD415-436
Cytoskeleton
lcsh:QH301-705.5
Actin
Ion channel
Phospholipase C
Chemistry
Transmembrane protein
Cell biology
030104 developmental biology
lcsh:Biology (General)
030220 oncology & carcinogenesis
Calcium
Intracellular
Research Article
Cellular swelling and blebbing
Subjects
Details
- Language :
- English
- ISSN :
- 24055808
- Database :
- OpenAIRE
- Journal :
- Biochemistry and Biophysics Reports
- Accession number :
- edsair.doi.dedup.....4a5f9e397b4fe968d0b9bfd26ed3b960
- Full Text :
- https://doi.org/10.1016/j.bbrep.2016.11.005