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Ultraviolet-B sensitivities in Japanese lowland rice cultivars: cyclobutane pyrimidine dimer photolyase activity and gene mutation
- Source :
- Plantcell physiology. 45(12)
- Publication Year :
- 2005
-
Abstract
- There is a cultivar difference in the response to ultraviolet-B (UVB: 280-320 nm) in rice (Oryza sativa L.). Among Japanese lowland rice cultivars, Sasanishiki, a leading Japanese rice cultivar, is resistant to the damaging effects of UVB while Norin 1, a close relative, is less resistant. We found previously that Norin 1 was deficient in cyclobutane pyrimidine dimer (CPD) photorepair ability and suggested that the UVB sensitivity in rice depends largely on CPD photorepair ability. In order to verify that suggestion, we examined the correlation between UVB sensitivity and CPD photolyase activity in 17 rice cultivars of progenitors and relatives in breeding of UV-resistant Sasanishiki and UV-sensitive Norin 1. The amino acid at position 126 of the deduced amino acid sequence of CPD photolyase in cultivars including such as Norin 1 was found to be arginine, the CPD photolyase activities of which were lower. The amino acid at that position in cultivars including such as Sasanishiki was glutamine. Furthermore, cultivars more resistant to UVB were found to exhibit higher photolyase activities than less resistant cultivars. These results emphasize that single amino acid alteration from glutamine to arginine leads to a deficit of CPD photolyase activity and that CPD photolyase activity is one of the main factors determining UVB sensitivity in rice.
- Subjects :
- DNA, Complementary
Arginine
Physiology
Photochemistry
Ultraviolet Rays
education
Molecular Sequence Data
Lyases
Pyrimidine dimer
Plant Science
Gene mutation
Japanese rice
Japan
Gene Expression Regulation, Plant
Amino Acid Sequence
Photolyase
chemistry.chemical_classification
Oryza sativa
biology
Base Sequence
food and beverages
Oryza
Cell Biology
General Medicine
biology.organism_classification
Amino acid
Glutamine
Biochemistry
chemistry
Pyrimidine Dimers
Seedlings
Mutation
Subjects
Details
- ISSN :
- 00320781
- Volume :
- 45
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Plantcell physiology
- Accession number :
- edsair.doi.dedup.....4a5b5757bc1dd9137fcac0707f091d0d