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Polymerase chain reaction diagnosis in fungal keratitis caused by Alternaria alternata
- Source :
- American Journal of Ophthalmology. 133:398-399
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- PURPOSE: To contribute toward assessing the effectiveness of polymerase chain reaction as a rapid method in diagnosis of torpid keratitis caused by opportunistic fungi. METHODS: Interventional case report. A 50-year-old man with a corneal abscess in the right eye treated for a period of 6 months with different combinations of broad-spectrum antibiotics and steroids was referred to our center. Corneal scraping was taken for microbiological study, including classic cultures and polymerase chain reaction. Amplified DNA was sequenced to identify the pathogen. RESULTS: Polymerase chain reaction amplification was negative for Acanthamoeba species and positive for fungi. The sequence analysis showed Alternaria alternata as the causal agent in 24 hours. Cultures confirmed the identification in 10 days. CONCLUSION: Polymerase chain reaction amplification with subsequent DNA-typing was revealed to be a useful method for detection of ocular pathogens such as A. alternata involved in cases of torpid keratitis, even in the presence of broad-spectrum antimicrobial therapy.
- Subjects :
- Male
medicine.drug_class
Genes, Fungal
Antibiotics
Polymerase Chain Reaction
Alternaria alternata
law.invention
Keratitis
Microbiology
law
medicine
Humans
Fungal keratitis
Corneal Ulcer
DNA, Fungal
Polymerase chain reaction
Mycosis
biology
Gene Amplification
Alternaria
Fungi imperfecti
Middle Aged
biology.organism_classification
medicine.disease
Virology
Abscess
Acanthamoeba
Ophthalmology
Mycoses
Eye Infections, Fungal
Subjects
Details
- ISSN :
- 00029394
- Volume :
- 133
- Database :
- OpenAIRE
- Journal :
- American Journal of Ophthalmology
- Accession number :
- edsair.doi.dedup.....4a4eaf59fe63d3e6e3443fa038ad2690