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GC-MS-based metabolomics approach to diagnose depression in hepatitis B virus-infected patients with middle or old age
- Source :
- Aging (Albany NY)
- Publication Year :
- 2018
- Publisher :
- Impact Journals, 2018.
-
Abstract
- Depression is concomitantly presented in hepatitis B virus (HBV)-infected patients (HB). However, there is still no objective method to diagnose HBV-infected patients with depression (dHB). Therefore, in this study, a gas chromatography-mass spectrometry (GC-MS)-based metabolomic approach was employed to profile urine samples from 59 dHB and 52 HB (the training set) in order to identify urinary metabolite biomarkers for dHB. Then, 41 dHB and 35 HB (the testing set) were used to independently validate the diagnostic generalizability of these biomarkers. In total, 13 differential metabolites responsible for the discrimination between dHB and HB were identified. These differential urinary metabolites belonged mainly to Lipid metabolism and Amino acid metabolism. A panel consisting of six urinary metabolite biomarkers (ethanolamine, azelaic acid, histidine, threitol, 2,4-dihydroxypyrimidine and levulinic acid) was identified. This panel was capable of distinguishing dHB from HB with an area under the receiver operating characteristic curve (AUC) of 0.986 in the training set. Moreover, this panel could classify blinded samples from the testing set with an AUC of 0.933. These findings indicated that the GC-MS-based metabolomics approach could be a useful tool in the clinical diagnosis of dHB, and the identified biomarkers were helpful for future developing an objective diagnostic method for dHB.
- Subjects :
- 0301 basic medicine
Adult
Male
Aging
medicine.medical_specialty
Urinary system
Metabolite
Urine
medicine.disease_cause
Gastroenterology
Gas Chromatography-Mass Spectrometry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Metabolomics
Internal medicine
medicine
Humans
Amino Acids
Aged
Hepatitis B virus
Receiver operating characteristic
business.industry
Depression
Cell Biology
Middle Aged
Hepatitis B
030104 developmental biology
chemistry
Biomarker (medicine)
biomarker
Female
Gas chromatography–mass spectrometry
business
hepatitis B virus
030217 neurology & neurosurgery
Biomarkers
Research Paper
metabolomic
Subjects
Details
- Language :
- English
- ISSN :
- 19454589
- Volume :
- 10
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Aging (Albany NY)
- Accession number :
- edsair.doi.dedup.....4a3e164fa30bd783abbbfe515fd18808