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Profiling of CD4(+) T Cells with Epigenetic Immune Lineage Analysis
- Source :
- The Journal of Immunology; Vol 186, The Journal of Immunology
- Publication Year :
- 2011
- Publisher :
- AMER ASSOC IMMUNOLOGISTS, 2011.
-
Abstract
- Proper transcriptional control of pro- and anti-inflammatory responses of the immune system is important for a fine-tuned balance between protection and tolerance. Emerging evidence suggests a key role for epigenetic regulation in governing the Th cell differentiation, where effector cytokines direct the overall immune response. In this study, we describe a method to pinpoint the location of isolated human CD4+ T cells on any T cell effector axis based on specific CpG methylation of cytokine and transcription factor loci. We apply the method on CD4+ cells obtained from rheumatoid arthritis and multiple sclerosis patients and show that synovial fluid infiltrating CD4+ T cells are committed toward both Th1 and regulatory T cell phenotype, whereas the Th2 response is suppressed. Furthermore, we show that the IL-17A gene is regulated by promoter methylation and that Th17 commitment is not a common feature in the inflamed joints of rheumatoid arthritis patients. We conclude that the method described in this paper allows for accurate profiling of Th lineage commitment in ex vivo-isolated CD4+ T cells.
- Subjects :
- CD4-Positive T-Lymphocytes
Multiple Sclerosis
Regulatory T cell
T cell
Immunology
Biology
Epigenesis, Genetic
Arthritis, Rheumatoid
TCIRG1
03 medical and health sciences
Interleukin 21
Th2 Cells
0302 clinical medicine
Immune system
medicine
Humans
Immunology and Allergy
Cytotoxic T cell
Cell Lineage
IL-2 receptor
Cells, Cultured
030304 developmental biology
0303 health sciences
Gene Expression Profiling
Interleukin-17
DNA Methylation
Th1 Cells
3. Good health
Cell biology
medicine.anatomical_structure
DNA methylation
Cytokines
030217 neurology & neurosurgery
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 00221767
- Volume :
- 186
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....4a3561aa5f83777d5d1f9b8dbc5799c9
- Full Text :
- https://doi.org/10.4049/jimmunol.1000960