Back to Search
Start Over
Optimal temporal–spatial fluorescence techniques for phenotyping nitrogen status in oilseed rape
- Source :
- Journal of Experimental Botany. 71:6429-6443
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Nitrogen (N) fertilizer maximizes the growth of oilseed rape (Brassica napus L.) by improving photosynthetic performance. Elucidating the dynamic relationship between fluorescence and plant N status could provide a non-destructive diagnosis of N status and the breeding of N-efficient cultivars. The aim of this study was to explore the impacts of different N treatments on photosynthesis at a spatial–temporal scale and to evaluate the performance of three fluorescence techniques for the diagnosis of N status. One-way ANOVA and linear discriminant analysis were applied to analyze fluorescence data acquired by a continuous excitation chlorophyll fluorimeter (OJIP transient analysis), pulse amplitude-modulated chlorophyll fluorescence (PAM-ChlF), and multicolor fluorescence (MCF) imaging. The results showed that the maximum quantum efficiency of PSII photochemistry (Fv/Fm) and performance index for photosynthesis (PIABS) of bottom leaves were sensitive to N status at the bolting stage, whereas the red fluorescence/far-red fluorescence ratio of top leaves was sensitive at the early seedling stage. Although the classification of N treatments by the three techniques achieved comparable accuracies, MCF imaging showed the best potential for early diagnosis of N status in field phenotyping because it had the highest sensitivity in the top leaves, at the early seedling stage. The findings of this study could facilitate research on N management and the breeding of N-efficient cultivars.
- Subjects :
- Chlorophyll
0106 biological sciences
0301 basic medicine
Nitrogen
Physiology
Brassica
Plant Science
Photosynthesis
01 natural sciences
Fluorescence
03 medical and health sciences
chemistry.chemical_compound
Fluorometer
Cultivar
Chlorophyll fluorescence
biology
Brassica napus
biology.organism_classification
Plant Leaves
Plant Breeding
Horticulture
030104 developmental biology
chemistry
Seedling
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14602431 and 00220957
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....4a330d1faee1d2d29c77a2d59c4bd26e