Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting

Authors :: Yves Le-Vern
Nathalie Winter
Marion Rambault
Emilie Doz-Deblauwe
Anca Dorhoi
Rachana Borkute
Aude Remot
Infectiologie et Santé Publique (UMR ISP)
Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Institute of Immunology
Friedrich-Loeffler-Institut (FLI)
Faculty of Mathematics and Natural Sciences
Universität Greifswald - University of Greifswald
Agence Nationale de la Recherche under the Carnot Program France Future Elevage
grant from the Institut de l’Élevage (Idele) Company (CIFRE N° 2019/0776)
FLI Intramural funds
Sven Brandau
Anca Dorhoi
Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Source :: Methods in Molecular Biology ISBN: 9781071610596, Myeloid-Derived Suppressor Cells, Sven Brandau; Anca Dorhoi. Myeloid-Derived Suppressor Cells, 2236, Humana Press, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1060-2_16⟩
Publication Year :: 2020
Publisher :: Springer US, 2020.
Abstract: International audience; Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.

ISBN :: 978-1-07-161059-6
ISBNs :: 9781071610596
Database :: OpenAIRE
Journal :: Methods in Molecular Biology ISBN: 9781071610596, Myeloid-Derived Suppressor Cells, Sven Brandau; Anca Dorhoi. Myeloid-Derived Suppressor Cells, 2236, Humana Press, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1060-2_16⟩
Accession number :: edsair.doi.dedup.....4a1add80dff794a59ac5cb7cb21d1218

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