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Multiphoton microscopy: a personal historical review, with some future predictions
- Source :
- Journal of Biomedical Optics
- Publication Year :
- 2020
- Publisher :
- SPIE-Intl Soc Optical Eng, 2020.
-
Abstract
- The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described.
- Subjects :
- Paper
two-photon fluorescence
Microscope
Materials science
Biomedical Engineering
Nanotechnology
History, 21st Century
01 natural sciences
Multiphoton Fluorescence Microscopy
law.invention
010309 optics
Biomaterials
Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
Signal strength
law
Confocal microscopy
0103 physical sciences
Microscopy
Humans
focal modulation microscopy
image scanning microscopy
fungi
Pulse duration
Second-harmonic generation
History, 20th Century
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Microscopy, Fluorescence, Multiphoton
Multiphoton fluorescence microscope
Microscopy, Fluorescence
multiphoton microscopy
Forecasting
second-harmonic generation
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....49f3696a923b46fe5d46d121de4db744