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DNA methylation profiling of human chromosomes 6, 20 and 22
- Source :
- Nature genetics
- Publication Year :
- 2006
-
Abstract
- DNA methylation constitutes the most stable type of epigenetic modifications modulating the transcriptional plasticity of mammalian genomes. Using bisulfite DNA sequencing, we report high-resolution methylation reference profiles of human chromosomes 6, 20 and 22, providing a resource of about 1.9 million CpG methylation values derived from 12 different tissues. Analysis of 6 annotation categories, revealed evolutionary conserved regions to be the predominant sites for differential DNA methylation and a core region surrounding the transcriptional start site as informative surrogate for promoter methylation. We find 17% of the 873 analyzed genes differentially methylated in their 5′-untranslated regions (5′-UTR) and about one third of the differentially methylated 5′-UTRs to be inversely correlated with transcription. While our study was controlled for factors reported to affect DNA methylation such as sex and age, we did not find any significant attributable effects. Our data suggest DNA methylation to be ontogenetically more stable than previously thought.
- Subjects :
- Adult
Male
Transcription, Genetic
Chromosomes, Human, Pair 22
Bisulfite sequencing
Chromosomes, Human, Pair 20
Biology
Article
Epigenesis, Genetic
Evolution, Molecular
Mice
03 medical and health sciences
0302 clinical medicine
Epigenetics of physical exercise
Species Specificity
Genetics
Animals
Humans
Methylated DNA immunoprecipitation
Promoter Regions, Genetic
RNA-Directed DNA Methylation
Aged
030304 developmental biology
Epigenomics
Sex Characteristics
0303 health sciences
Age Factors
DNA Methylation
Middle Aged
Differentially methylated regions
Organ Specificity
030220 oncology & carcinogenesis
DNA methylation
Illumina Methylation Assay
Chromosomes, Human, Pair 6
CpG Islands
Female
5' Untranslated Regions
Subjects
Details
- Language :
- English
- ISSN :
- 15461718 and 10614036
- Volume :
- 38
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Nature genetics
- Accession number :
- edsair.doi.dedup.....49a263296dc3321b9fe70203a3d4fe5b