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Lanthanum-Doped Chitosan Hydrogels Promote the Apoptosis of Melanoma Cells by Bcl-2/Bax Pathway
- Source :
- ACS Applied Bio Materials. 1:1468-1477
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- The surgical resection of melanoma may cause skin wounds, and the remaining melanoma cells bring a great risk of tumor recurrence. To overcome the above problem, we for the first time constructed lanthanum-doped chitosan (La-CS) hydrogels with excellent wound healing and antitumor functions. The La element was uniformly dispersed within whole hydrogels, and part of La3+ ions reacted with CS to form La-CS complex. The complexation interaction between La3+ ions and CS significantly improve the La3+ release performances of La-CS hydrogels. The as-released La3+ ions from the composite hydrogels selectively inhibited the proliferation of B-16 melanoma cells, but showed lower toxic side effects to L929 skin fibroblast cells. Moreover, the La3+ ions triggered the apoptosis of B-16 cells through Bcl-2/Bax pathway, as confirmed by the Annexin Vand PI double staining, flow cytometry, and Western blot results. The in vivo tumor models of C57 mice revealed that the La-CS hydrogels had more significant relapse-inhibit...
- Subjects :
- inorganic chemicals
Biomedical Engineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Flow cytometry
Biomaterials
Western blot
Annexin
In vivo
medicine
integumentary system
medicine.diagnostic_test
Chemistry
Melanoma
Biochemistry (medical)
technology, industry, and agriculture
General Chemistry
021001 nanoscience & nanotechnology
medicine.disease
Molecular biology
0104 chemical sciences
Apoptosis
Self-healing hydrogels
0210 nano-technology
Wound healing
Subjects
Details
- ISSN :
- 25766422
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- ACS Applied Bio Materials
- Accession number :
- edsair.doi.dedup.....4994aaaf45f842fb27690106d88b9294