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TPP ionically cross-linked chitosan/PLGA microspheres for the delivery of NGF for peripheral nerve system repair
- Source :
- Carbohydrate polymers. 258
- Publication Year :
- 2020
-
Abstract
- To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared. The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %. Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm. The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days. The sustained release of NGF could promote PC12 cells differentiation and neurite growth in vitro. Moreover, in comparison with NGF solution without microencapsulation, TPP/Chitosan/PLGA-NGF microspheres enhanced sciatic nerve regeneration and prevented gastrocnemius muscle atrophy in rats. These results demonstrate the feasibility of using TPP/Chitosan/PLGA-NGF microspheres for neural tissue repair.
- Subjects :
- Male
Polymers and Plastics
Polymers
Surface Properties
macromolecular substances
02 engineering and technology
Matrix (biology)
010402 general chemistry
01 natural sciences
PC12 Cells
law.invention
Chitosan
Rats, Sprague-Dawley
chemistry.chemical_compound
Drug Delivery Systems
Polylactic Acid-Polyglycolic Acid Copolymer
Confocal microscopy
law
Nerve Growth Factor
Peripheral Nervous System
Spectroscopy, Fourier Transform Infrared
Materials Chemistry
Zeta potential
Neurites
Animals
Particle Size
Muscle, Skeletal
Ions
Neurons
Microscopy, Confocal
Organic Chemistry
technology, industry, and agriculture
Cell Differentiation
021001 nanoscience & nanotechnology
Sciatic Nerve
In vitro
Microspheres
0104 chemical sciences
Nerve Regeneration
Rats
PLGA
Nerve growth factor
Cross-Linking Reagents
nervous system
chemistry
Biophysics
Sciatic nerve
0210 nano-technology
Subjects
Details
- ISSN :
- 18791344
- Volume :
- 258
- Database :
- OpenAIRE
- Journal :
- Carbohydrate polymers
- Accession number :
- edsair.doi.dedup.....496a1cdca50bbc493df75703c5afeda8