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Herpes Simplex Virus Type 2 (HSV-2) Western Blot Confirmatory Testing Among Men Testing Positive for HSV-2 Using the Focus Enzyme-Linked Immunosorbent Assay in a Sexually Transmitted Disease Clinic
- Source :
- Sexually Transmitted Diseases. 32:771-777
- Publication Year :
- 2005
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2005.
-
Abstract
- The objective of this study was to define the positive predictive value (PPV) of the Focus herpes simplex virus type 2 (HSV-2) enzyme-linked immunosorbent assay (ELISA) in a low HSV-2 prevalence population and to develop a new test interpretation algorithm.HSV-2 Western blots were performed on sera from male sexually transmitted disease clinic patients testing HSV-2 ELISA-positive and used to define a new class of indeterminate HSV-2 ELISA result. HSV-2 Western blots were then prospectively performed on sequential sera with indeterminate HSV-2 ELISAs.Ninety-one (84%) of 108 HSV-2 ELISA-positive sera tested HSV-2 Western blot-positive. Western blot positivity was more common in men without herpes simplex virus type 1 (HSV-1) antibody than in those with HSV-1 antibody (93% vs 76%, P = 0.02) and in men with a history or clinical evidence of genital lesions (88% vs 80%, P = 0.30). Selectively raising the ELISA index value defining HSV-2 positivity from1.1 toor=3.0 either among HSV-1-positive men or among those without a history or clinical evidence of genital lesions increased the PPV toor=93%. Prospective evaluation of an algorithm incorporating HSV-1 serostatus found that 11 of 70 persons with indeterminate HSV-2 ELISAs were Western blot-positive.Clinicians should consider selectively using a higher index value to define Focus ELISA HSV-2 positivity based on either HSV-1 serostatus or clinical circumstances.
- Subjects :
- Adult
Male
Microbiology (medical)
Sexually transmitted disease
Adolescent
genetic structures
Herpesvirus 2, Human
viruses
Blotting, Western
Population
Sexually Transmitted Diseases
Enzyme-Linked Immunosorbent Assay
Dermatology
Antibodies, Viral
medicine.disease_cause
Ambulatory Care Facilities
Sensitivity and Specificity
Herpesviridae
Virus
Predictive Value of Tests
Alphaherpesvirinae
Prevalence
medicine
Humans
education
Aged
education.field_of_study
Herpes Genitalis
biology
business.industry
Public Health, Environmental and Occupational Health
Middle Aged
biology.organism_classification
Virology
Infectious Diseases
Herpes simplex virus
Immunology
biology.protein
Viral disease
Antibody
business
Algorithms
Subjects
Details
- ISSN :
- 01485717
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Sexually Transmitted Diseases
- Accession number :
- edsair.doi.dedup.....495f36844f2b3326add4a0f16723ad85