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Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment
- Source :
- Enzyme Research, Vol 2019 (2019), Repositório Institucional do INPA, Instituto Nacional de Pesquisas da Amazônia (INPA), instacron:INPA, Enzyme Research
- Publication Year :
- 2019
- Publisher :
- Hindawi Limited, 2019.
-
Abstract
- Microbial lipases are prominent biocatalysts able to catalyze a wide variety of reactions in aqueous and nonaqueous media. In this work, filamentous fungi isolated from leaves decomposed in an aquatic environment were screened for lipase production with hydrolytic activity and esterification. Agar plates with Tween 20 and Rhodamine B were used for selection, while submerged cultures with olive oil were subsequently used to select 38 filamentous fungi. Trichoderma harzianum, Fusarium solani, Trichoderma harzianum F5, and Penicillium sp. F36 were grown in six different culture media. F. solani presented the highest lipase production (2.37 U/mL) with esterification activity of 0.07 U/mL using medium composed of (g.L−1) KH2PO4 1.00, MgSO4 H2O 1.123, and CuSO4 0.06. Supplementation of this culture medium with organic nitrogen sources increased lipase production by 461.3% using tryptone and by 419.4% using yeast extract. Among the vegetable oils from the Amazon region, degummed cotton oil induced lipase production up to 8.14 U/mL. The lipase produced by F. solani F61 has great potential to application in conventional processes and biodiesel production by transesterification of vegetable oils, as well as food industries in the production of fatty acid esters by hydrolysis and esterification.
- Subjects :
- Article Subject
Rhodamine B
Filamentous Fungus
Fungus Culture
Biochemistry
Hypocrea Lixii
lcsh:Biochemistry
chemistry.chemical_compound
Yeast extract
Fusarium Solani
lcsh:QD415-436
Food science
Controlled Study
Plant Leaf
Lipase
Molecular Biology
Olive Oil
Vegetable Oil
lcsh:QH301-705.5
Aquatic Environment
Decomposition
biology
Esterification
Organic Nitrogen
Penicillium
Trichoderma harzianum
food and beverages
Polysorbate 20
Transesterification
Fungal Enzyme
Fungus Isolation
biology.organism_classification
Nonhuman
Agar
chemistry
lcsh:Biology (General)
Tryptone
Fungus Growth
Biodiesel production
biology.protein
Triacylglycerol Lipase
Enzyme Synthesis
Fusarium solani
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 20900414 and 20900406
- Volume :
- 2019
- Database :
- OpenAIRE
- Journal :
- Enzyme Research
- Accession number :
- edsair.doi.dedup.....495356a3833afa63a7815573b5c6af08