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Altered Renal Sodium Transporter Expression in an Animal Model of Type 2 Diabetes Mellitus
- Source :
- Journal of Korean Medical Science
- Publication Year :
- 2007
- Publisher :
- Korean Academy of Medical Sciences, 2007.
-
Abstract
- Hemodynamic factors play an important role in the development and/or progression of diabetic nephropathy. We hypothesized that renal sodium transporter dysregulation might contribute to the hemodynamic alterations in diabetic nephropathy. Otsuka Long Evans Tokushima Fatty (OLETF) rats were used as an animal model for type 2 diabetes. Long Evans Tokushima (LETO) rats were used as controls. Renal sodium transporter regulation was investigated by semiquantitative immunoblotting and immunohistochemistry of the kidneys of 40-week-old animals. The mean serum glucose level in OLETF rats was increased to 235+/-25 mg/dL at 25 weeks, and the hyperglycemia continued up to the end of 40 weeks. Urine protein/ creatinine ratios were 10 times higher in OLETF rats than in LETO rats. At 40th week, the abundance of the epithelial sodium channel (ENaC) beta-subunit was increased in OLETF rats, but the abundance of the ENaC gamma-subunit was decreased. No significant differences were observed in the ENaC alpha-subunit or other major sodium transporters. Immunohistochemistry for the ENaC beta-subunit showed increased immunoreactivity in OLETF rats, whereas the ENaC gamma-subunit showed reduced immunoreactivity in these rats. In OLETF rats, ENaC beta-subunit upregulation and ENaC gamma-subunit downregulation after the development of diabetic nephropathy may reflect an abnormal sodium balance.
- Subjects :
- Blood Glucose
Male
Epithelial sodium channel
medicine.medical_specialty
Sodium-Hydrogen Exchangers
Sodium-Potassium-Chloride Symporters
Rats, Inbred OLETF
Sodium
Immunoblotting
chemistry.chemical_element
Type 2 diabetes
Kidney
Diabetic nephropathy
chemistry.chemical_compound
Diabetes mellitus
Internal medicine
medicine
Animals
Diabetic Nephropathies
Thiazide Sensitive Nacl Cotransporter
Epithelial Sodium Channels
Solute Carrier Family 12, Member 1
Creatinine
Sodium-Hydrogen Exchanger 3
urogenital system
business.industry
Kidney metabolism
General Medicine
Bumetanide Sensitive NaK2Cl Cotransporter
medicine.disease
Epithelial Sodium Channel
Immunohistochemistry
Rats
Disease Models, Animal
Endocrinology
medicine.anatomical_structure
Diabetes Mellitus, Type 2
chemistry
Hypertension
Original Article
business
Subjects
Details
- ISSN :
- 10118934
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Journal of Korean Medical Science
- Accession number :
- edsair.doi.dedup.....49415524532bf4d6e15634da28e4babe