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Simultaneous removal of nitrogen and phosphorous by heterotrophic nitrification-aerobic denitrification of a metal resistant bacterium Pseudomonas putida strain NP5
- Source :
- Bioresource technology. 285
- Publication Year :
- 2019
-
Abstract
- A novel strain NP5 with efficient heterotrophic nitrification, aerobic denitrification and phosphorus accumulation ability was isolated and identified as Pseudomonas putida strain NP5. The removed ammonium and phosphate were mainly converted into intracellular components by assimilation, and negligible nitrification intermediates and N2O were accumulated during heterotrophic nitrification. In addition, the optimal conditions for nutrient removal were: succinate as carbon source, C/N 10, P/N 0.2, temperature 30 °C, salinity 0% and shaking speed 160 rpm. Besides, strain NP5 possessed an exceptional heavy metal and nanoparticles resistance. Cr6+ was found to be the most toxic among the tested metals, and it could be removed simultaneously. Moreover, an obvious phosphorus release was observed under anaerobic condition, and repeated exposure to the anaerobic/aerobic conditions could significantly improve the nutrient removal. Furthermore, the successful expression of key enzymes for nitrogen and phosphorous removal provided additional evidence for possibility of simultaneous nitrification, denitrification and phosphorus removal.
- Subjects :
- 0106 biological sciences
Environmental Engineering
Denitrification
Nitrogen
Heterotroph
Bioengineering
010501 environmental sciences
01 natural sciences
chemistry.chemical_compound
010608 biotechnology
Aerobic denitrification
Ammonium
Waste Management and Disposal
Nitrites
0105 earth and related environmental sciences
biology
Renewable Energy, Sustainability and the Environment
Chemistry
Pseudomonas putida
Heterotrophic Processes
Phosphorus
General Medicine
Phosphate
biology.organism_classification
Nitrification
Aerobiosis
Metals
Environmental chemistry
Anaerobic exercise
Subjects
Details
- ISSN :
- 18732976
- Volume :
- 285
- Database :
- OpenAIRE
- Journal :
- Bioresource technology
- Accession number :
- edsair.doi.dedup.....49405e14179a238ddee4104013679920