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Deciphering Chemical Mediators Regulating Specialized Metabolism in a Symbiotic Cyanobacterium

Authors :
Julia Krumbholz
Keishi Ishida
Martin Baunach
Jonna E. Teikari
Magdalena M. Rose
Severin Sasso
Christian Hertweck
Elke Dittmann
Source :
Angewandte Chemie International Edition. 61
Publication Year :
2022
Publisher :
Wiley, 2022.

Abstract

Genomes of cyanobacteria feature a variety of cryptic biosynthetic pathways for complex natural products, but the peculiarities limiting the discovery and exploitation of the metabolic dark matter are not well understood. Here we describe the discovery of two cell density-dependent chemical mediators, nostoclide and nostovalerolactone, in the symbiotic model strain Nostoc punctiforme, and demonstrate their pronounced impact on the regulation of specialized metabolism. Through transcriptional, bioinformatic and labeling studies we assigned two adjacent biosynthetic gene clusters to the biosynthesis of the two polyketide mediators. Our findings provide insight into the orchestration of specialized metabolite production and give lessons for the genomic mining and high-titer production of cyanobacterial bioactive compounds.

Details

ISSN :
15213773 and 14337851
Volume :
61
Database :
OpenAIRE
Journal :
Angewandte Chemie International Edition
Accession number :
edsair.doi.dedup.....493bf9b5ac61e88ca55330f5592ed7a5
Full Text :
https://doi.org/10.1002/anie.202204545