Back to Search
Start Over
The malignant histiocytosis sarcoma virus, a recombinant of Harvey murine sarcoma virus and Friend mink cell focus-forming virus, has acquired myeloid transformation specificity by alterations in the long terminal repeat
- Source :
- Journal of Virology. 64:369-378
- Publication Year :
- 1990
- Publisher :
- American Society for Microbiology, 1990.
-
Abstract
- The malignant histiocytosis sarcoma virus (MHSV), in contrast to other viruses with the ras oncogene, induces acute histiocytosis in newborn and adult mice. Molecular structure and function studies were initiated to determine the basis of its unique macrophage-transforming potential. Characterization of the genomic structure showed that the virus evolved by recombination of the Harvey murine sarcoma virus (Ha-MuSV) and a virus of the Friend-mink cell focus-forming virus family. Structural analysis of MHSV showed two regions of the genome that are basically different from the Ha-MuSV: (i) the ras gene, which is altered by a point mutation in codon 181 leading to a Cys----Ser substitution of the p21 protein, and (ii) the U3 region of the long terminal repeat, which is largely derived from F-MCFV and contains a deletion of one direct repeat as well as a duplication of an altered enhancer-like region. Biological studies of Ha-MuSV, MHSV, and recombinants between the two viruses show that the U3 region of the MHSV long terminal repeat is essential for the malignancy and specificity of the disease. A contributing role of the ras point mutation in determining macrophage specificity, however, cannot be excluded.
- Subjects :
- Genes, Viral
viruses
Molecular Sequence Data
Restriction Mapping
Immunology
Harvey murine sarcoma virus
Viral transformation
Biology
Transfection
Microbiology
Virus
Cell Line
Sarcoma Viruses, Murine
Mice
Viral Envelope Proteins
Mink Cell Focus-Inducing Viruses
Sequence Homology, Nucleic Acid
Virology
Murine leukemia virus
Animals
Direct repeat
Amino Acid Sequence
Cloning, Molecular
Codon
Repetitive Sequences, Nucleic Acid
Recombination, Genetic
Mice, Inbred BALB C
Base Sequence
Point mutation
Provirus
biology.organism_classification
Long terminal repeat
Leukemia Virus, Murine
Cell Transformation, Neoplastic
Genes, ras
Insect Science
DNA, Viral
Histiocytosis
Spleen
Research Article
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....491d1971d46340403532aced552b6fed