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Activity of the RhoU/Wrch1 GTPase is critical for cranial neural crest cell migration

Authors :
Sandrine Faure
Pascal de Santa Barbara
Cécile Notarnicola
Linda Guémar
Nathalie Morin
Philippe Fort
Emmanuel Vignal
Centre de recherche en Biologie Cellulaire (CRBM)
Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)
Muscle et pathologies
Université Montpellier 1 (UM1)-IFR3
Université Montpellier 1 (UM1)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp)
Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Centre de recherches de biochimie macromoléculaire (CRBM)
Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-IFR122-Centre National de la Recherche Scientifique (CNRS)
Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH)
VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)
Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Dubois, Frederic
Source :
Developmental Biology, Developmental Biology, Elsevier, 2011, 350 (2), pp.451-463. ⟨10.1016/j.ydbio.2010.12.011⟩, Developmental Biology, 2011, 350 (2), pp.451-463. ⟨10.1016/j.ydbio.2010.12.011⟩
Publication Year :
2011
Publisher :
HAL CCSD, 2011.

Abstract

International audience; The neural crest (NC) is a stem cell-like population that arises at the border of neural and non-neural ectoderm. During development, NC undergoes an epithelio-mesenchymal transition (EMT), i.e. loss of epithelial junctions and acquisition of pro-migratory properties, invades the entire embryo and differentiates into a wide diversity of terminal tissues. We have studied the implication of Rho pathways in NC development and previously showed that RhoV is required for cranial neural crest (CNC) cell specification. We show here that the non-canonical Wnt response rhoU/wrch1 gene, closely related to rhoV, is also expressed in CNC cells but at later stages. Using both gain- and loss-of-function experiments, we demonstrate that the level of RhoU expression is critical for CNC cell migration and subsequent differentiation into craniofacial cartilages. In in vitro cultures, RhoU activates pathways that cooperate with PAK1 and Rac1 in epithelial adhesion, cell spreading and directional cell migration. These data support the conclusion that RhoU is an essential regulator of CNC cell migration.

Details

Language :
English
ISSN :
00121606 and 1095564X
Database :
OpenAIRE
Journal :
Developmental Biology, Developmental Biology, Elsevier, 2011, 350 (2), pp.451-463. ⟨10.1016/j.ydbio.2010.12.011⟩, Developmental Biology, 2011, 350 (2), pp.451-463. ⟨10.1016/j.ydbio.2010.12.011⟩
Accession number :
edsair.doi.dedup.....49156ee021364279ab28f3fc2232c94a
Full Text :
https://doi.org/10.1016/j.ydbio.2010.12.011⟩