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Activity of the RhoU/Wrch1 GTPase is critical for cranial neural crest cell migration
- Source :
- Developmental Biology, Developmental Biology, Elsevier, 2011, 350 (2), pp.451-463. ⟨10.1016/j.ydbio.2010.12.011⟩, Developmental Biology, 2011, 350 (2), pp.451-463. ⟨10.1016/j.ydbio.2010.12.011⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- International audience; The neural crest (NC) is a stem cell-like population that arises at the border of neural and non-neural ectoderm. During development, NC undergoes an epithelio-mesenchymal transition (EMT), i.e. loss of epithelial junctions and acquisition of pro-migratory properties, invades the entire embryo and differentiates into a wide diversity of terminal tissues. We have studied the implication of Rho pathways in NC development and previously showed that RhoV is required for cranial neural crest (CNC) cell specification. We show here that the non-canonical Wnt response rhoU/wrch1 gene, closely related to rhoV, is also expressed in CNC cells but at later stages. Using both gain- and loss-of-function experiments, we demonstrate that the level of RhoU expression is critical for CNC cell migration and subsequent differentiation into craniofacial cartilages. In in vitro cultures, RhoU activates pathways that cooperate with PAK1 and Rac1 in epithelial adhesion, cell spreading and directional cell migration. These data support the conclusion that RhoU is an essential regulator of CNC cell migration.
- Subjects :
- rho GTP-Binding Proteins
Xenopus
Cell
Ectoderm
MESH: rac GTP-Binding Proteins
Chick Embryo
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Xenopus Proteins
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy
Xenopus laevis
0302 clinical medicine
Cranial neural crest
Cell Movement
Rho GTPases
MESH: Animals
RhoU
MESH: Cell Movement
MESH: Xenopus Proteins
Migration
ComputingMilieux_MISCELLANEOUS
0303 health sciences
education.field_of_study
Wnt signaling pathway
Cell Polarity
Neural crest
Cell migration
MESH: Chick Embryo
rac GTP-Binding Proteins
Cell biology
medicine.anatomical_structure
Neural Crest
MESH: Cell Polarity
MESH: Focal Adhesion Kinase 2
MESH: p21-Activated Kinases
Population
RAC1
[SDV.CAN]Life Sciences [q-bio]/Cancer
Biology
03 medical and health sciences
MESH: Xenopus laevis
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
medicine
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
education
Molecular Biology
030304 developmental biology
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Cell Biology
MESH: rho GTP-Binding Proteins
MESH: Neural Crest
Focal Adhesion Kinase 2
[SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis
p21-Activated Kinases
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 00121606 and 1095564X
- Database :
- OpenAIRE
- Journal :
- Developmental Biology, Developmental Biology, Elsevier, 2011, 350 (2), pp.451-463. ⟨10.1016/j.ydbio.2010.12.011⟩, Developmental Biology, 2011, 350 (2), pp.451-463. ⟨10.1016/j.ydbio.2010.12.011⟩
- Accession number :
- edsair.doi.dedup.....49156ee021364279ab28f3fc2232c94a
- Full Text :
- https://doi.org/10.1016/j.ydbio.2010.12.011⟩