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Effect of Inhaled Interleukin-5 on Number and Activity of Eosinophils in Circulation from Asthmatics
- Source :
- Clinical Immunology. 91:163-169
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- The aim of the present study was to examine the effects of interleukin-5 (IL-5) inhalation on changes in the activity and number of circulating eosinophils, as well as concentrations of serum total IgE, in allergic asthmatics. A randomized double-blind, placebo-controlled study design was employed in which each subject acted as his or her own control. Eight nonsmoking patients with allergic asthma were administered recombinant human IL-5 by nebulization. Total white blood cell counts and differentials, as well as concentrations of ECP and total IgE in serum, were determined before and at 2, 24, and 48 h after inhalation. Our results demonstrated that eosinophil numbers increased from baseline (3.6 +/- 1.1 x 10(5)/ml) to 6.3 +/- 1.2 x 10(5)/ml (P0.01) at 24 h and to 5.7 +/- 0.9 x 10(5)/ml (P0.01) at 48 h after IL-5 inhalation in asthmatics. Accompanying this significantly increased blood eosinophilia were significantly elevated serum ECP levels. Compared with baseline (6.3 +/- 1.1 ng/ml), ECP levels increased with time following IL-5 inhalation, reaching 17.6 +/- 2.8 ng/ml (P0.01) at 24 h and remaining elevated at 48 h (18.1 +/- 2.9 ng/ml, P0.01). IL-5 inhalation had no significant effect on levels of serum total IgE, however. These findings provide direct evidence that nebulized IL-5 not only induces a significant blood eosinophilia but also results in the activation of circulating eosinophils. Our data further support the importance of IL-5 in the pathogenesis of bronchial asthma in humans.
- Subjects :
- Adult
Male
medicine.medical_specialty
Allergy
Immunology
Immunoglobulin E
Ribonucleases
Double-Blind Method
Internal medicine
White blood cell
Administration, Inhalation
Humans
Immunology and Allergy
Medicine
Eosinophilia
Interleukin 5
Eosinophil cationic protein
Inhalation
biology
business.industry
Blood Proteins
Eosinophil Granule Proteins
Middle Aged
Eosinophil
medicine.disease
Asthma
Recombinant Proteins
Eosinophils
medicine.anatomical_structure
Endocrinology
biology.protein
Female
Interleukin-5
medicine.symptom
business
Subjects
Details
- ISSN :
- 15216616
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Clinical Immunology
- Accession number :
- edsair.doi.dedup.....491218ef300d70e93730701df24061b7