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The influence of surface chemistry on the kinetics and thermodynamics of bacterial adhesion
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
- Publication Year :
- 2018
- Publisher :
- Nature Publishing Group UK, 2018.
-
Abstract
- This work is concerned with investigating the effect of substrate hydrophobicity and zeta potential on the dynamics and kinetics of the initial stages of bacterial adhesion. For this purpose, bacterial pathogens Staphylococcus aureus and Escherichia coli O157:H7 were inoculated on the substrates coated with thin thiol layers (i.e., 1-octanethiol, 1-decanethiol, 1-octadecanethiol, 16-mercaptohexadecanoic acid, and 2-aminoethanethiol hydrochloride) with varying hydrophobicity and surface potential. The time-resolved adhesion data revealed a transformation from an exponential dependence to a square root dependence on time upon changing the substrate from hydrophobic or hydrophilic with a negative zeta potential value to hydrophilic with a negative zeta potential for both pathogens. The dewetting of extracellular polymeric substances (EPS) produced by E. coli O157:H7 was more noticeable on hydrophobic substrates, compared to that of S. aureus, which is attributed to the more amphiphilic nature of staphylococcal EPS. The interplay between the timescale of EPS dewetting and the inverse of the adhesion rate constant modulated the distribution of E. coli O157:H7 within microcolonies and the resultant microcolonial morphology on hydrophobic substrates. Observed trends in the formation of bacterial monolayers rather than multilayers and microcolonies rather than isolated and evenly spaced bacterial cells could be explained by a colloidal model considering van der Waals and electrostatic double-layer interactions only after introducing the contribution of elastic energy due to adhesion-induced deformations at intercellular and substrate-cell interfaces. The gained knowledge is significant in the context of identifying surfaces with greater risk of bacterial contamination and guiding the development of novel surfaces and coatings with superior bacterial antifouling characteristics.
- Subjects :
- 0301 basic medicine
Staphylococcus aureus
Surface Properties
030106 microbiology
lcsh:Medicine
Context (language use)
02 engineering and technology
Escherichia coli O157
Article
Bacterial Adhesion
Biofouling
03 medical and health sciences
Amphiphile
Monolayer
Zeta potential
Dewetting
Sulfhydryl Compounds
lcsh:Science
Multidisciplinary
Chemistry
Extracellular Polymeric Substance Matrix
lcsh:R
Substrate (chemistry)
Adhesion
021001 nanoscience & nanotechnology
Kinetics
Biophysics
Thermodynamics
lcsh:Q
0210 nano-technology
Hydrophobic and Hydrophilic Interactions
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....48f25954c8054edd793f6ea3877840b2