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p53 codon 72 genotype affects apoptosis by cytosine arabinoside in blood leukocytes
- Source :
- King's College London
-
Abstract
- A wide difference in the susceptibility to undergo in vitro apoptosis exists among individuals, and this fact has potential implications in predicting the in vivo response to apoptotic agents, such as those employed in chemotherapy. In this report, we addressed the question whether the natural variability at p53 locus (the proline-arginine substitution at codon 72) affects the capacity of peripheral-blood mononuclear cells from healthy subjects to undergo in vitro apoptosis in response to the cytotoxic drug cytosine arabinoside. We found that cells from subjects carrying the arginine/arginine genotype undergo in vitro apoptosis at a higher extent in comparison to those from arginine/proline subjects. This finding suggests that naturally occurring genetic variability at p53 gene explains part of the inter-individual difference in the in vitro susceptibility to a chemotherapeutic drug, thus resulting as an eligible predictor marker of in vivo response to chemotherapy and its adverse effects.
- Subjects :
- Adult
Antimetabolites, Antineoplastic
Arginine
Genotype
medicine.medical_treatment
Biophysics
Apoptosis
Biology
Biochemistry
Peripheral blood mononuclear cell
chemistry.chemical_compound
In vivo
medicine
Leukocytes
Humans
Polymorphism
Codon
Cytosine arabinoside
Molecular Biology
Chemotherapy
Analysis of Variance
Cytarabine
Genetic Variation
Cell Biology
Exons
In vitro
p53 codon 72, Polymorphism, Apoptosis, Cytosine arabinoside
p53 codon 72
chemistry
Immunology
Cancer research
Tumor Suppressor Protein p53
Cytosine
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- King's College London
- Accession number :
- edsair.doi.dedup.....48c2932050ff02ba3437befeab9491fc