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Molecular analysis of V617F mutation in Janus kinase 2 gene of breast cancer patients
- Source :
- Saudi Journal of Biological Sciences, Saudi Journal of Biological Sciences, Vol 26, Iss 6, Pp 1123-1128 (2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- Background: Breast cancer is a multifactorial disease with the highest frequency in females. Genetic and environmental factors can cause mutation in several genes like tyrosine kinase, JAK2 gene which may initiate cancer. Molecular analysis of mutations in the JAK2 gene along with determination of environmental, clinical and haematological risk factors associated with breast cancer patients is need of hour to improve patient's healthcare. Somatic JAK2 valine-to-phenylalanine (617 codon) mutation is one of the widely prevalent mutations. Methods: Blood was collected from seventy breast cancer patients after their consent. The questionnaire included risk factors, age group, locality, number of children, tumor type, family history, time of initial diagnosis, no of cycles/month, water conditions and exposure to radiations. Molecular analysis were carried out from genomic DNA using Sanger sequencing and allele-specific PCR to check the V617F point mutation. Results: The breast cancer risk factors includes unfiltered water (68.57%), urban (58.57%), menopause (55.71%), family history of cancer (18.57%), tumor grades (II, 37.14% and III, 35.71%), consanguineous marriages (44.28%) and having more than 3–4 children (45.71%). Prevalence of breast cancer was higher after the age of 35 and maximum at 35–50. In allele-specific PCR of 70 patients, 25 patients were wild type (229 bp), 25 patients were with partially deleted gene (200 bp), and 20 patient had shown no or less than 40 bp size fragments. In Sanger's sequencing of 70 BC cases, 18% were found to be positive for V617F point mutation, including 6 homozygous (T/T) and 7 heterozygous (G/T) mutations at nucleotide position 1849 in exon 14 of the JAK2 gene. Conclusions: Environmental and clinical risk factors were associated with breast cancer which can be overcome by improving awareness of associated risks, health facilities and reducing stress. Keywords: Breast cancer, JAK2, V617F mutation, Allele-specific PCR, Sanger’s sequencing
- Subjects :
- 0106 biological sciences
0301 basic medicine
Oncology
medicine.medical_specialty
V617F mutation
medicine.disease_cause
01 natural sciences
Article
03 medical and health sciences
Exon
symbols.namesake
Breast cancer
Internal medicine
medicine
Family history
Gene
lcsh:QH301-705.5
Sanger sequencing
Mutation
business.industry
Point mutation
Cancer
Allele-specific PCR
medicine.disease
Sanger’s sequencing
030104 developmental biology
lcsh:Biology (General)
JAK2
symbols
General Agricultural and Biological Sciences
business
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 22137106 and 1319562X
- Volume :
- 26
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Saudi Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....48c16896a430e22d83c2c6cca7a54303