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Comparison of microbiota in the cloaca, colon, and magnum of layer chicken
- Source :
- PLoS ONE, PLoS ONE, Vol 15, Iss 8, p e0237108 (2020)
- Publication Year :
- 2020
-
Abstract
- Anatomically terminal parts of the urinary, reproductive, and digestive systems of birds all connect to the cloaca. As the feces drain through the cloaca in chickens, the cloacal bacteria were previously believed to represent those of the digestive system. To investigate similarities between the cloacal microbiota and the microbiota of the digestive and reproductive systems, microbiota inhabiting the colon, cloaca, and magnum, which is a portion of the chicken oviduct of 34-week-old, specific-pathogen-free hens were analyzed using a 16S rRNA metagenomic approach using the Ion torrent sequencer and the Qiime2 bioinformatics platform. Beta diversity via unweighted and weighted unifrac analyses revealed that the cloacal microbiota was significantly different from those in the colon and the magnum. Unweighted unifrac revealed that the cloacal microbiota was distal from the microbiota in the colon than from the microbiota in the magnum, whereas weighted unifrac revealed that the cloacal microbiota was located further away from the microbiota in the magnum than from the microbiota inhabiting the colon. Pseudomonas spp. were the most abundant in the cloaca, whereas Lactobacillus spp. and Flavobacterium spp. were the most abundant species in the colon and the magnum. The present results indicate that the cloaca contains a mixed population of bacteria, derived from the reproductive, urinary, and digestive systems, particularly in egg-laying hens. Therefore, sampling cloaca to study bacterial populations that inhabit the digestive system of chickens requires caution especially when applied to egg-laying hens. To further understand the physiological role of the microbiota in chicken cloaca, exploratory studies of the chicken's cloacal microbiota should be performed using chickens of different ages and types.
- Subjects :
- 0301 basic medicine
Oviducts
Poultry
Cloaca
Lactobacillus
Medicine and Health Sciences
Gamefowl
Animal Anatomy
education.field_of_study
Multidisciplinary
biology
Gastrointestinal Microbiome
Eukaryota
UniFrac
embryonic structures
Vertebrates
Medicine
Female
Anatomy
Research Article
animal structures
Colon
Science
030106 microbiology
Population
Zoology
digestive system
Flavobacterium
Birds
03 medical and health sciences
Pseudomonas
Animal Genital Anatomy
Animals
education
Feces
Bacteria
Gut Bacteria
Organisms
Biology and Life Sciences
biology.organism_classification
16S ribosomal RNA
Gastrointestinal Tract
stomatognathic diseases
030104 developmental biology
Fowl
Amniotes
Metagenome
Chickens
Digestive System
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....48b3f00788a536b7b535e9940b36e61e