Back to Search
Start Over
Computational classification of MocR transcriptional regulators into subgroups as a support for experimental and functional characterization
- Source :
- Bioinformation
- Publication Year :
- 2019
- Publisher :
- Biomedical informatics Ltd, 2019.
-
Abstract
- MocR bacterial transcriptional regulators are a subfamily within the GntR family. The MocR proteins possess an N-terminal domain containing the winged Helix-Turn-Helix (wHTH) motif and a C-terminal domain whose architecture is homologous to the fold type-I pyridoxal 5'-phosphate (PLP) dependent enzymes and whose archetypical protein is aspartate aminotransferase (AAT). The ancestor of the fold type-I PLP dependent super-family is considered one of the earliest enzymes. The members of this super-family are the product of evolution which resulted in a diversified protein population able to catalyze a set of reactions on substrates often containing amino groups. The MocR regulators are activators or repressors of gene control within many metabolic pathways often involving PLP enzymes. This diversity implies that MocR specifically responds to different classes of effector molecules. Therefore, it is of interest to compare the AAT domains of MocR from six bacteria phyla. Multi dimensional scaling and cluster analyses suggested that at least three subgroups exist within the population that reflects functional specialization rather than taxonomic origin. The AAT-domains of the three clusters display variable degree of similarity to different fold type-I PLP enzyme families. The results support the hypothesis that independent fusion events generated at least three different MocR subgroups.
- Subjects :
- Pyridoxal 5'-phosphate
Subfamily
pyridoxal 5’-phosphate
Population
Repressor
Computational biology
Biology
03 medical and health sciences
chemistry.chemical_compound
Structural bioinformatics
0302 clinical medicine
aspartate aminotransferase
mocr
structural bioinformatics
multidimensional scaling analysis
cluster analysis
education
Pyridoxal
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
education.field_of_study
Effector
General Medicine
Metabolic pathway
Enzyme
chemistry
030217 neurology & neurosurgery
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Bioinformation
- Accession number :
- edsair.doi.dedup.....48a0baa25b427519660561caa6732a7e