Back to Search
Start Over
Complex challenges of estimating the age and vitality of muscle wounds : a study with matrix metalloproteinases and their inhibitors on animal and human tissue samples
- Source :
- International Journal of Legal Medicine
- Publication Year :
- 2021
-
Abstract
- The estimation of wound age and wound vitality is a recurring task in forensic routine work and has been subject of forensic research for a long time. By now, an unrestrictedly reliable marker or set of markers has not been found. In a study on myocardial infarctions, matrix metalloproteinases (MMP) 2 and 9 as well as tissue inhibitor of matrix metalloproteinases 1 (TIMP-1) were detected immunohistochemically in mechanically wounded myocardium (ECG electrodes, vessel ligations). Against this background, the potency of MMP-9, MMP-2, and TIMP-1 as markers for the estimation of wound age and wound vitality was tested in a broad approach with human tissue samples drawn during autopsies and with an animal model, the isolated perfused Langendorff heart. The study comprised samples of injured human skeletal muscle, injured human myocardium, rats’ hearts with vital wounds, and rats’ hearts with postmortem-inflicted wounds that were all stained immunohistochemically. The results showed great scattering, leading to the conclusion that MMP-2, MMP-9, and TIMP-1 are not suitable for wound age estimation. Merely the results for TIMP-1 suggested that this marker might be able to differentiate between vital and postmortem-inflicted wounds. With a view to the promising results of the preceding study, the results underline the necessity to test possible markers of wound age/wound vitality on a large and diverse sample set.
- Subjects :
- Male
0301 basic medicine
Pathology
medicine.medical_specialty
Langendorff heart
Medizin
Skeletal muscle
Matrix metalloproteinase
Human myocardium
Vitality
Pathology and Forensic Medicine
Wound vitality
03 medical and health sciences
0302 clinical medicine
Animal model
medicine
Animals
Humans
030216 legal & forensic medicine
Muscle, Skeletal
Forensic Pathology
Wound Healing
Tissue Inhibitor of Metalloproteinase-1
integumentary system
business.industry
Myocardium
Routine work
Immunohistochemistry
Rats
030104 developmental biology
medicine.anatomical_structure
Matrix Metalloproteinase 9
Matrix Metalloproteinase 2
Wounds and Injuries
Original Article
Female
business
Wound age
Biomarkers
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- International Journal of Legal Medicine
- Accession number :
- edsair.doi.dedup.....4889196a319a5cbca7a15ae67044cf9c