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Establishment and characterization of two new cell lines from the mosquito Armigeres subalbatus (Coquillett) (Diptera: Culicidae)
- Source :
- In Vitro Cellular & Developmental Biology - Animal. 51:672-679
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Armigeres subalbatus (Coquillett) is a medically important mosquito and a model species for immunology research. We successfully established two cell lines from the neonate larvae of A. subalbatus using two different media. To our knowledge, this is the first report of an established Armigeres mosquito cell line. The cell lines, designated as Ar-3 and Ar-13, consist of adherent and diploid cells with compact colonies. Both these cell lines grow slowly after passage at a split ratio of 1:5 and a population doubling time of 2.7 and 3.0 d, respectively. Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to confirm that these lines correspond to the species of origin and are clearly distinct from seven other insect cell lines. Furthermore, reverse-transcription PCR was used to demonstrate that the Ar-3 cell line is susceptible to the Japanese encephalitis virus and two insect flaviviruses associated with Culex and Aedes mosquitoes but relatively insensitive to dengue virus. These data indicate that the newly established cell lines are cellular models of A. subalbatus as well as beneficial tools for the propagation of viruses associated with the Armigeres mosquito.
- Subjects :
- Culex
media_common.quotation_subject
Primary Cell Culture
Insect
Dengue virus
medicine.disease_cause
Virus
Cell Line
medicine
Animals
media_common
Encephalitis Virus, Japanese
Aedes
Larva
biology
Reverse Transcriptase Polymerase Chain Reaction
Flavivirus
fungi
Cell Biology
General Medicine
Dengue Virus
Japanese encephalitis
biology.organism_classification
medicine.disease
Virology
Random Amplified Polymorphic DNA Technique
Culicidae
Cell culture
Female
Developmental Biology
Subjects
Details
- ISSN :
- 1543706X and 10712690
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- In Vitro Cellular & Developmental Biology - Animal
- Accession number :
- edsair.doi.dedup.....4879371b8f1a59a245d3a6582e45288d